Rabbit Anti-Cdk4 Recombinant Antibody (VS13-YC198)
CAT#: VS13-YC198
The Rabbit Anti-Cdk4 Recombinant Antibody is specific for human and mouse Cdk4. Cdk4 engages in cyclin-component binding and activities affecting diseases like skin melanoma through gated complex interactions. The anti-Cdk4 antibody can be used for Western blot (1/1000-1/2000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry-paraffin (1/1000), Immunoprecipitation (1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli, nuclear membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6024/1704_B1_1_cr5811e29350250_selected.jpg
Normal Tissue
Figure 2 IHC staining of human ovary
Immunohistochemical staining of human ovary shows strong nuclear positivity in ovarian stroma cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_4_7_selected.jpg
Normal Tissue
Figure 3 IHC staining of human endometrium
Immunohistochemical staining of human endometrium shows moderate nuclear positivity in cells in endometrial stroma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69405/163411_B_5_3_selected.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Goblet cells Staining: Low Intensity: Moderate Quantity: <25% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_5_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (HBEC3-KT, LHCN-M2, RH-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135446-CDK4
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Specifications
- Immunogen
- Recombinant mouse Cdk4, aa 180-303.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse Cdk4
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, IP
- Conjugate
- NONE
- Related Disease
- carcinoma, familial melanoma, glioblastoma, obesity, and type 2 diabetes mellitus
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/1000
Immunoprecipitation (IP): 1/50
Target
- Alternative Names
- Crk3
- Gene ID
- 12567
- UniProt ID
- P11802
- Long Name
- cyclin dependent kinase 4
- Sequence Similarities
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
- Cellular Localization
- Nucleus; Cytoplasm; Cell membrane
- Protein Refseq
- NP_000066.1
- Function
- Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
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Related Diseases
Bladder Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
Related Signaling Pathways
p53 Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CDK4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0519CT | Recombinant Mouse anti-Human CDK4 Monoclonal antibody (EML005) | ICC, IF, WB | |
| VS3-FY346 | Recombinant Rabbit Anti-CDK4 Antibody (clone R04-4I5) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-FY347 | Recombinant Rabbit Anti-CDK4 Antibody (clone R04-8I5) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-FY348 | Recombinant Rabbit Anti-CDK4 Antibody (clone R07-4G7) | WB, ICC, IF | Rabbit IgG |
| VS4-WK100 | Mouse Anti-CDK4 Recombinant Antibody (VS4-WK100) | ELISA, WB, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0113MZ | Chicken Anti-CDK4 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0630 | Hi-Affi™ Rabbit Anti-CDK4 Recombinant Antibody (clone DS630AB) | ICC, IF, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC569 | AbPlus™ Anti-CDK4 Magnetic Beads (VS-0724-YC569) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY404 | Anti-CDK4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1317 | Anti-Mouse CDK4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1316 | Anti-Human CDK4 Immunohistochemistry Kit | IHC |
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