Mouse Anti-CSF2 Recombinant Antibody (clone 196) (CAT#: FAMAB-0330JF)
Recombinant monoclonal antibody specially binds to human colony stimulating factor 2. Clone 196 is a mouse monoclonal antibody that can be useful in immunoassays.
Specific Inquiry
IHC
Figure 1 CSF2 immunostaining on representative sections of normal urothelium .
The non-neoplastic urothelium shows very low expression of CSF2.
Lee, Y. Y., Wu, W. J., Huang, C. N., Li, C. C., Li, W. M., Yeh, B. W., ... & Li, C. F. (2016). CSF2 overexpression is associated with STAT5 phosphorylation and poor prognosis in patients with urothelial carcinoma. Journal of Cancer, 7(6), 711.
WB
Figure 2 CSF-2 Is Actively Secreted by Stem Cells in Response to Various Injury SignalsIn VitroandIn Vivo.
MSCs were cultured with or without serum for 48 h.
Park, S. R., Cho, A., Kim, J. W., Lee, H. Y., & Hong, I. S. (2019). A novel endogenous damage signal, CSF-2, activates multiple beneficial functions of adipose tissue-derived mesenchymal stem cells. Molecular Therapy, 27(6), 1087-1100.
IF
Figure 3 CSF-2 Promotes the Differentiation and Migratory Capacities of MSCs In Vitro.
he effects of CSF-2 on osteoblast differentiation were determined by alizarin redstaining.
Park, S. R., Cho, A., Kim, J. W., Lee, H. Y., & Hong, I. S. (2019). A novel endogenous damage signal, CSF-2, activates multiple beneficial functions of adipose tissue-derived mesenchymal stem cells. Molecular Therapy, 27(6), 1087-1100.
ELISA
Figure 4 Increased CSF-2 concentrations by TCA treatment in the serum samples were also detected by ELISA.
β-actin was used as the internal control. The results represent the mean±SD from threeindependent experiments.
Park, S. R., Cho, A., Kim, J. W., Lee, H. Y., & Hong, I. S. (2019). A novel endogenous damage signal, CSF-2, activates multiple beneficial functions of adipose tissue-derived mesenchymal stem cells. Molecular Therapy, 27(6), 1087-1100.
Specifications
- Immunogen
- Recombinant hGMCSF
- Host Species
- Mouse
- Derivation
- Mouse bacteria display scFv library
- Type
- Mouse IgG
- Specificity
- Human CSF2
- Species Reactivity
- Human
- Clone
- 196
- Applications
- WB, ELISA, IHC, IF
- Related Disease
- Acute myocardial infarction; Systemic inflammatory response syndrome
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The CSF2 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence.
Target
- Alternative Names
- CSF2; colony stimulating factor 2 (granulocyte-macrophage); GMCSF; granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim; granulocyte macrophage-colony stimulating factor
- Gene ID
- 1437
- UniProt ID
- P04141
Related Resources
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H44 | Anti-Human CSF2 Recombinant Antibody (Lenzilumab) | Neut, ELISA, IF, IP, FuncS, FC, IHC | IgG1 - kappa |
| TAB-189CL | Human Anti-CSF2 Recombinant Antibody (TAB-189CL) | ELISA | Human IgG1 |
| TAB-177CT | Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody (KB002) | ELISA, Neut | Human antibody |
| TAB-187CT-S(P) | Human Anti-CSF2 Recombinant Antibody; scFv Fragment (TAB-187CT-S(P)) | ELISA, FC | Human scFv |
| TAB-187CT-F(E) | Human Anti-CSF2 Recombinant Antibody; Fab Fragment (TAB-187CT-F(E)) | ELISA, FC | Human Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-183CT | Mouse Anti-CSF2 Recombinant Antibody (TAB-183CT) | ELISA, FC, WB, Block | Mouse IgG |
| TAB-185CT | Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody (4K21) | ELISA, Neut | |
| TAB-183CT-S(P) | Mouse Anti-CSF2 Recombinant Antibody; scFv Fragment (TAB-183CT-S(P)) | ELISA, FC, WB, Block | Mouse scFv |
| TAB-185CT-S(P) | Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody scFv Fragment (4K21) | ELISA, Neut | |
| TAB-185CT-F(E) | Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody Fab Fragment (4K21) | ELISA, Neut |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-184CT | Human Anti-CSF2 Recombinant Antibody (TAB-184CT) | ELISA, FC, WB, Block | Chimeric (Mouse/Human) IgG1, κ |
| TAB-184CT-S(P) | Human Anti-CSF2 Recombinant Antibody; scFv Fragment (TAB-184CT-S(P)) | ELISA, FC, WB, Block | Human scFv |
| TAB-184CT-F(E) | Human Anti-CSF2 Recombinant Antibody; Fab Fragment (TAB-184CT-F(E)) | ELISA, FC, WB, Block | Chimeric (Mouse/Human) Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-186CT | Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody (hGM4/1-34) | ELISA, Neut | Humanized antibody |
| TAB-186CT-S(P) | Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody scFv Fragment (hGM4/1-34) | ELISA, Neut | Humanized antibody |
| TAB-186CT-F(E) | Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody Fab Fragment (hGM4/1-34) | ELISA, Neut | Humanized antibody |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-511CQ | Recombinant Mouse Anti-CSF2 Antibody (CBL568) | Neut | IgG1 |
| NEUT-529CQ | Mouse Anti-CSF2 Recombinant Antibody (clone CBL521) | WB, Neut | Mouse IgG1 |
| NEUT-530CQ | Rabbit Anti-CSF2 Recombinant Antibody (clone CBL687) | Neut | Rabbit IgG |
| NEUT-531CQ | Mouse Anti-Csf2 Recombinant Antibody (clone CBL308) | Neut, ELISA | Mouse IgG2a |
| NEUT-532CQ | Mouse Anti-Csf2 Recombinant Antibody (clone CBL501) | WB, CyTOF®, ICC, ICFC, Neut | Mouse IgG2b |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-512CQ | Rat Anti-CSF2 Recombinant Antibody (clone 22E9), Biotin | Block, ELISA, WB | Rat IgG2a |
| NEUT-514CQ | Rat Anti-CSF2 Recombinant Antibody (clone 31G6) | Block, ELISA, WB | Rat IgG1 |
| NEUT-525CQ | Rat Anti-Csf2 Recombinant Antibody (clone CBL251) | Inhib | Rat IgG2a |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4580 | Hi-Affi™ Rabbit Anti-CSF2 Recombinant Antibody (clone TH92DS) | WB | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0136-FY | Human Anti-CSF2 Recombinant Antibody (clone MSB-0010628) | Inhib | Humanized IgG1, κ |
| HPAB-1090-FY | Human Anti-CSF2 Recombinant Antibody (HPAB-1090-FY) | ELISA | Human IgG |
| HPAB-1091-FY | Human Anti-CSF2 Recombinant Antibody (HPAB-1091-FY) | ELISA | Human IgG |
| HPAB-1092-FY | Human Anti-CSF2 Recombinant Antibody (HPAB-1092-FY) | ELISA | Human IgG |
| HPAB-1093-FY | Human Anti-CSF2 Recombinant Antibody (HPAB-1093-FY) | ELISA | Human IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0014-FY-S(P) | Human Anti-CSF2 Recombinant Antibody (clone hGM4/35); scFv Fragment | ELISA | Human scFv |
| PSBC-337 | Human Anti-CSF2 Recombinant Antibody (clone F1); scFv Fragment | ELISA, FC, Neut | Human scFv |
| PSBC-338 | Mouse Anti-CSF2 Recombinant Antibody (clone 4D4); scFv Fragment | ELISA, FC, Neut | Mouse scFv |
| HPAB-N0052-YC-S(P) | Human Anti-CSF2 Recombinant Antibody; scFv Fragment (HPAB-N0052-YC-S(P)) | ELISA, Block | Humanized scFv |
| PABJ-0112-S(P) | Human Anti-CSF2 Recombinant Antibody (clone EV1007); scFv Fragment | ELISA, Neut | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0136-FY-F(E) | Human Anti-CSF2 Recombinant Antibody (clone MSB-0010628); Fab Fragment | Inhib | Humanized Fab |
| PFBC-337 | Human Anti-CSF2 Recombinant Antibody (clone F1); Fab Fragment | ELISA, FC, Neut | Human Fab |
| PFBC-338 | Mouse Anti-CSF2 Recombinant Antibody (clone 4D4); Fab Fragment | ELISA, FC, Neut | Mouse Fab |
| HPAB-1090-FY-S(P) | Human Anti-CSF2 Recombinant Antibody; scFv Fragment (HPAB-1090-FY-S(P)) | ELISA | Human scFv |
| HPAB-1091-FY-S(P) | Human Anti-CSF2 Recombinant Antibody; scFv Fragment (HPAB-1091-FY-S(P)) | ELISA | Human scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-259 | Afuco™ Anti-CSF2 ADCC Recombinant Antibody (Namilumab), ADCC Enhanced | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
| AFC-TAB-H44 | Afuco™ Anti-CSF2 ADCC Recombinant Antibody (Lenzilumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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