Rabbit Anti-DNMT1 Recombinant Antibody (clone R08-4C7)
CAT#: VS3-XY3334
The product is a recombinant rabbit antibody binding with DNMT1 (DNA methyltransferase 1), which associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. It recognizes and binds to human DNMT1. It also has cross-reactivity against mouse and rat DNMT1. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5876/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5876/613_E12_1_red_green.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2694/8662_A_4_6.jpg
Normal Tissue
Figure 4 Placenta
Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2694/8662_A_1_7.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000130816-DNMT1
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat DNMT1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-4C7
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Narcolepsy, Autosomal Dominant
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI
- Gene ID
- 1786
- UniProt ID
- P26358
- Long Name
- DNA methyltransferase 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys58, Lys88, Lys134, Lys586, Lys666, Lys928, Lys961, Lys981, Lys997, Lys1323, Lys1415, Lys1483, and Lys1572 (NX_P26358 [NX_P26358-1])
Modification sites at PhosphoSitePlus (P26358)
Glycosylation from GlyGen (P26358) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001124295.1; XP_047278844.1
- Function
- Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-XY3334. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "DNMT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-310 | Human Anti-DNMT1 Recombinant Antibody (clone D420) | ELISA, IP-MS | Human IgG |
| MOB-0562CT | Recombinant Mouse anti-Human DNMT1 Monoclonal antibody (EML149) | FC, ICC, IF, IHC-P, WB | |
| MRO-0486-CN | Rabbit Anti-DNMT1 Recombinant Antibody (clone JF09-89) | WB, IF, IHC | Rabbit IgG |
| ZG-0083U | Rabbit Anti-DNMT1 Recombinant Antibody (clone 1A9) | ELISA, IHC, IF | Rabbit IgG |
| VS3-XY590 | Mouse Anti-DNMT1 Recombinant Antibody (clone 7C11A2) | ELISA, WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0164MZ | Chicken Anti-DNMT1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1028 | Hi-Affi™ Rabbit Anti-DNMT1 Recombinant Antibody (clone DS1028AB) | FC, ICC, IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC314 | CytoStream™ Mouse Anti-DNMT1 Recombinant Antibody (VS13-YC314) | WB, IHC-P, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2045 | Anti-DNMT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2046 | Anti-Mouse DNMT1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: WB, ELISA, FC, IHC, IP
Application: WB, ELISA, Neut, FuncS
Application: ELISA, WB
Application: ELISA, Neut, FuncS
Application: ELISA, WB
Application: WB, IHC-Fr, IHC-P, ICC, IF
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
