Rabbit Anti-DNMT1 Recombinant Antibody (clone R08-4C7) (CAT#: VS3-XY3334)
The product is a recombinant rabbit antibody binding with DNMT1 (DNA methyltransferase 1), which associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. It recognizes and binds to human DNMT1. It also has cross-reactivity against mouse and rat DNMT1. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5876/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5876/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5876/613_E12_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5876/613_E12_1_red_green.jpg
Figure 3 Testis
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2694/8662_A_4_6.jpg
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2694/8662_A_4_6.jpg
Figure 4 Placenta
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2694/8662_A_1_7.jpg
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2694/8662_A_1_7.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000130816-DNMT1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000130816-DNMT1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat DNMT1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-4C7
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Narcolepsy, Autosomal Dominant
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI
- Gene ID
- 1786
- UniProt ID
- P26358
- Long Name
- DNA methyltransferase 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys58, Lys88, Lys134, Lys586, Lys666, Lys928, Lys961, Lys981, Lys997, Lys1323, Lys1415, Lys1483, and Lys1572 (NX_P26358 [NX_P26358-1])
Modification sites at PhosphoSitePlus (P26358)
Glycosylation from GlyGen (P26358) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001124295.1; XP_047278844.1
- Function
- Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-310 | Human Anti-DNMT1 Recombinant Antibody (clone D420) | ELISA, IP-MS | Human IgG |
| MOB-0562CT | Recombinant Mouse anti-Human DNMT1 Monoclonal antibody (EML149) | FC, ICC/IF, IHC-P, WB | |
| MRO-A1926-YJ | Mouse Anti-DNMT1 Recombinant Antibody (clone CBS388) | WB, ELISA | Mouse IgG2b |
| ZG-0083U | Rabbit Anti-DNMT1 Recombinant Antibody (clone 1A9) | ELISA, IHC, IF | Rabbit IgG |
| VS3-FY460 | Recombinant Rabbit Anti-DNMT1 Antibody (clone R05-3K1) | WB, ICC, IF, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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