Rabbit Anti-DNMT1 Recombinant Antibody (VS13-YC315)
CAT#: VS13-YC315
The Rabbit Anti-DNMT1 Recombinant Antibody is specific for human and mouse DNMT1. DNMT1 encodes DNA methyltransferase, maintaining methylation patterns critical for gene regulation, impacted in conditions like developmental abnormalities and tumor pathologies. The anti-DNMT1 antibody can be used for Western blot (1/500-1/2000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry-paraffin (1/50-1/200).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5876/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5876/613_E12_1_red_green.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2694/8662_A_4_6.jpg
Normal Tissue
Figure 4 Placenta
Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2694/8662_A_1_7.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000130816-DNMT1
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Specifications
- Immunogen
- Recombinant human Dnmt1, aa 1506-1549.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse DNMT1
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P
- Conjugate
- NONE
- Related Disease
- Narcolepsy, Autosomal Dominant
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Target
- Alternative Names
- DNA Methyltransferase 1; DNA (Cytosine-5-)-Methyltransferase 1; CXXC-Type Zinc Finger Protein 9; DNA Methyltransferase HsaI; DNA MTase HsaI; EC 2.1.1.37; M.HsaI; CXXC9;
- Gene ID
- 1786
- UniProt ID
- P26358
- Long Name
- DNA Methyltransferase 1
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_001124295.1; XP_047278844.1
- Function
- Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "DNMT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-310 | Human Anti-DNMT1 Recombinant Antibody (clone D420) | ELISA, IP-MS | Human IgG |
| MOB-0562CT | Recombinant Mouse anti-Human DNMT1 Monoclonal antibody (EML149) | FC, ICC, IF, IHC-P, WB | |
| MRO-A1926-YJ | Mouse Anti-DNMT1 Recombinant Antibody (clone CBS388) | WB, ELISA | Mouse IgG2b |
| VS3-XY590 | Mouse Anti-DNMT1 Recombinant Antibody (clone 7C11A2) | ELISA, WB, IHC | Mouse IgG1 |
| VS3-FY460 | Recombinant Rabbit Anti-DNMT1 Antibody (clone R05-3K1) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0164MZ | Chicken Anti-DNMT1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1028 | Hi-Affi™ Rabbit Anti-DNMT1 Recombinant Antibody (clone DS1028AB) | FC, ICC, IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC314 | CytoStream™ Mouse Anti-DNMT1 Recombinant Antibody (VS13-YC314) | WB, IHC-P, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2045 | Anti-DNMT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2046 | Anti-Mouse DNMT1 Immunohistochemistry Kit | IHC |
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