Rabbit Anti-FASN Recombinant Antibody (clone 2A5)
CAT#: ZG-0665J
This product is a rabbit antibody that recognizes FASN.
Tested Data
Gene Expression
IF
Figure 1 Rabbit Anti-FASN Antibody (ZG-0665J) in IF
Immunofluorescence staining of Hela Cells with this product at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
FC
Figure 2 Rabbit Anti-FASN Antibody (ZG-0665J) in FC
Overlay histogram showing A549 cells stained with this product (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6461/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5192/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6461/155195_B_8_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6461/155195_A_4_6.jpg
Normal Tissue
Figure 5 Breast
Adipocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6461/155195_B_3_4.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6461/155195_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169710-FASN
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Specifications
- Immunogen
- A synthesized peptide derived from human FASN
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human FASN
- Species Reactivity
- Human
- Clone
- 2A5
- Applications
- ELISA, IF, FC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunofluorescence, Flow Cytometry.
IF:1:20-1:200, FC:1:20-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "FASN"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3035z | Mouse Anti-FASN Recombinant Antibody (clone 36H1) | WB, ICC, IF | Mouse IgG1, κ |
| MOB-0705CT | Recombinant Mouse anti-Human FASN Monoclonal antibody (3F2-1F3) | WB | |
| MRO-0571-CN | Rabbit Anti-FASN Recombinant Antibody (clone JJ0939) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-1855-CN | Rabbit Anti-FASN Polyclonal Antibody (MRO-1855-CN) | WB, IF, IHC | Rabbit IgG |
| VS3-CJ985 | Mouse Anti-FASN Recombinant Antibody (VS3-CJ985) | WB, ICC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1254 | Hi-Affi™ Rabbit Anti-FASN Recombinant Antibody (clone DS1254AB) | WB, IHC, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX416 | Mouse Anti-FASN Recombinant Antibody (clone 497CT15.2.5) | WB, ICC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY98 | AbPlus™ Anti-FASN Magnetic Beads (2F9-B2-B6) | IP, Protein Purification | |
| VS-0724-YC513 | AbPlus™ Anti-FASN Magnetic Beads (VS-0724-YC513) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY816 | Anti-FASN Immunohistochemistry Kit | IHC | |
| VS-0525-XY2452 | Anti-Mouse FASN Immunohistochemistry Kit | IHC | |
| VS-0525-XY2451 | Anti-Human FASN Immunohistochemistry Kit | IHC |
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