Anti-FGF2 Antibody Prodrug, Protease Activated (VS-1025-YC148)
CAT#: VS-1025-YC148
The Anti-FGF2 Antibody Prodrug, Protease Activated (VS-1025-YC148) is an antibody prodrug comprised of a recombinant human antibody targeting human FGF2, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to FGF2 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to FGF2 effectively. FGF2, also known as Basic Fibroblast Growth Factor (bFGF), is a powerful secreted growth factor that acts as a key component in the tumor microenvironment. It is a major stimulator of both tumor cell proliferation and, critically, angiogenesis (new blood vessel growth) by binding to and activating its corresponding FGFR receptors on endothelial cells. High levels of FGF2 expression in tumors are often associated with aggressive disease, poor prognosis, and resistance to therapies targeting other growth factors like VEGFA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/641_G7_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human caudate
Immunohistochemical staining of human caudate shows nuclear positivity in glia.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/65502/1336_684_200x200.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/640_G7_1_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/642_G7_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_9_5.jpg
Normal Tissue
Figure 6 Caudate
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_8_7.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_8_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_9_5.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, fHDF/TERT166, hTERT-HME1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138685-FGF2
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human FGF2
- Target
- FGF2
- Species Reactivity
- Human
- Isotype
- IgG
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
- Alternative Names
- Fibroblast Growth Factor 2; Heparin-Binding Growth Factor 2; HBGF-2; FGF-2; BFGF; FGFB; Prostatropin
- Gene ID
- 2247
- UniProt ID
- P09038
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COA
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