Recombinant Human Anti-human FGF2 Antibody (CAT#: MHH-357)
Recombinant Human Antibody specifically reacts with Human FGF2, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-FGF2 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-FGF2 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/641_G7_4_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/641_G7_4_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/640_G7_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/640_G7_1_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/642_G7_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/642_G7_1_red_green.jpg
Figure 2 IHC staining of human caudate
(Immunohistochemical staining of human caudate shows nuclear positivity in glia.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/65502/1336_684_200x200.jpg
(Immunohistochemical staining of human caudate shows nuclear positivity in glia.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/65502/1336_684_200x200.jpg
Figure 5 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_9_5.jpg
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_9_5.jpg
Figure 6 Caudate
(Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_8_7.jpg
(Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_8_7.jpg
Figure 7 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_8_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_8_3.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_9_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_9_5.jpg
Figure 9 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, fHDF/TERT166, hTERT-HME1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138685-FGF2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138685-FGF2
Specifications
- Immunogen
- Human fibroblast growth factor 2 (basic)
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Human fibroblast growth factor 2 (basic)
- Species Reactivity
- Human
- Applications
- Antibody assay: Radioimmunoassay; Immunofluorescence; Functional Study
Product Property
- Purity
- >95% as judged by SDS-polyacrylamide gel electrophoresis
- Storage
- ≤1 year at -20°C.
Target
- Alternative Names
- FGF2; fibroblast growth factor 2 (basic); BFGF; FGFB; FGF-2; HBGF-2; fibroblast growth factor 2; prostatropin; heparin-binding growth factor 2; basic fibroblast growth factor bFGF
- Gene ID
- 2247
- UniProt ID
- P09038
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-357-F(E) | Recombinant Anti-human FGF2 Antibody Fab Fragment | IF, FC, FuncS | Fab |
| MHH-357-F(E) | Recombinant Human Anti-human FGF2 Antibody Fab Fragment | IP, IF, Biosensors, FuncS | Fab |
| HPAB-0164-WJ-F(E) | Mouse Anti-FGF2 Recombinant Antibody (clone E12); Fab Fragment | ELISA | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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