Mouse Anti-FGF2 Recombinant Antibody (clone E12) (CAT#: HPAB-0164-WJ)
This product is a mouse antibody targeting human basic fibroblast growth factor (bFGF/FGF2). This antibody is capable of fibroblast growth factor receptor competition binding human basic fibroblast cell growth factor (bFGF), can block the bFGF and tumor cell surface expression of the FGFR binding, blocking bFGF / FGFR pathway of signal transduction, and thus can effectively inhibit tumor cell proliferation.
Specific Inquiry
ELISA
Figure 1 Identification of purified antibody.
(B) Measurement of relative affinity constant of mAbE12 antibody. Results are presented as the mean ± standard deviation. bFGF, basic fibroblast growth factor; mAb, monoclonal antibody.
Yang, Y., Luo, Z., Qin, Y., Zhou, Y. U., Gong, L., Huang, J., & Wang, H. (2017). Production of bFGF monoclonal antibody and its inhibition of metastasis in Lewis lung carcinoma. Molecular medicine reports, 16(4), 4015-4021.
WB
Figure 2 The AKT/GSK3-β/Snail pathway was suppressed by mAbE12.
A dose-dependent decrease of p-AKT was observed in the mAbE12 group. The expression of Snail was downregulated and the expression of E-cadherin was upregulated in the mAbE12 group. β-actin served as a control. AKT, protein kinase B; p-, phosphorylated; Snail, snail family transcriptional repressor 1; GSK3-β, glycogen synthase kinase 3 β; mAb, monoclonal antibody.
Yang, Y., Luo, Z., Qin, Y., Zhou, Y. U., Gong, L., Huang, J., & Wang, H. (2017). Production of bFGF monoclonal antibody and its inhibition of metastasis in Lewis lung carcinoma. Molecular medicine reports, 16(4), 4015-4021.
IHC
Figure 3 Effect of mAbE12 on Lewis lung carcinoma cell migration.
Results are presented as the mean ± standard deviation. mAb, monoclonal antibody; IgG, immunoglobulin G.
Yang, Y., Luo, Z., Qin, Y., Zhou, Y. U., Gong, L., Huang, J., & Wang, H. (2017). Production of bFGF monoclonal antibody and its inhibition of metastasis in Lewis lung carcinoma. Molecular medicine reports, 16(4), 4015-4021.
Inhib
Figure 4 Effect of mAb on LLC metastasis and growth in vivo.
(C) Tumor growth curve of mAbE12 and PBS control group. Data are presented as the mean ± SD. *P<0.05 vs. PBS control. mAb, monoclonal antibody; LLC, Lewis lung carcinoma; SD, standard deviation; PBS, phosphate-buffered saline.
Yang, Y., Luo, Z., Qin, Y., Zhou, Y. U., Gong, L., Huang, J., & Wang, H. (2017). Production of bFGF monoclonal antibody and its inhibition of metastasis in Lewis lung carcinoma. Molecular medicine reports, 16(4), 4015-4021.
Specifications
- Immunogen
- Recombinant human basic fibroblast growth factor (bFGF)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human FGF2
- Species Reactivity
- Human
- Clone
- E12
- Applications
- ELISA
- Related Disease
- Lung cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The FGF2 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Inhibition.
For ELISA: Recombinant human bFGF (50 ng/well) was used to coat microtiter ELISA wells. The ELISA wells were blocked with 5% non-fat dry milk in PBS buffer containing 0.5% Tween-20 for 1 h at 37°C, bFGF incubated with purified antibody (purified with Protein G Sepharose, 1:400,000) at 37°C for 1 h, then HRP-conjugated goat anti-mouse antibody (1:8,000) at 37°C for 40 min. The absorbance values were measured at 450 µm on an automatic plate reader.
Target
- Alternative Names
- FGF2; fibroblast growth factor 2 (basic); BFGF; FGFB; FGF-2; HBGF-2; fibroblast growth factor 2; prostatropin
- Gene ID
- 2247
- UniProt ID
- P09038
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-357-F(E) | Recombinant Anti-human FGF2 Antibody Fab Fragment | IF, FC, FuncS | Fab |
| MHH-357-F(E) | Recombinant Human Anti-human FGF2 Antibody Fab Fragment | IP, IF, Biosensors, FuncS | Fab |
| HPAB-0164-WJ-F(E) | Mouse Anti-FGF2 Recombinant Antibody (clone E12); Fab Fragment | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-357-S(P) | Recombinant Anti-human FGF2 Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| MHH-357-S(P) | Recombinant Human Anti-human FGF2 Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| HPAB-0164-WJ-S(P) | Mouse Anti-FGF2 Recombinant Antibody (clone E12); scFv Fragment | ELISA | Mouse scFv |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-279CL | Human Anti-FGF2 Recombinant Antibody (TAB-279CL) | ELISA | Human IgG |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-048WM | Anti-Human FGF2 Recombinant Antibody (3F12E7) | ELISA, WB, IHC, FuncS | |
| TAB-048WM-S(P) | Anti-Human FGF2 Recombinant Antibody scFv Fragment (3F12E7) | ELISA, FuncS | |
| TAB-048WM-F(E) | Anti-Human FGF2 Recombinant Antibody Fab Fragment (3F12E7) | ELISA, FuncS |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-049WM | Anti-Human FGF2 Recombinant Antibody (6) | ELISA, WB | Human Antibody |
| TAB-049WM-S(P) | Anti-Human FGF2 Recombinant Antibody scFv Fragment (6) | ELISA, WB | Human Antibody |
| TAB-049WM-F(E) | Anti-Human FGF2 Recombinant Antibody Fab Fragment (6) | ELISA, WB | Human Antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0725CT | Recombinant Mouse anti-Human FGF2 Monoclonal antibody (3D9) | ELISA | |
| MOB-094LC | Mouse Anti-FGF2 Recombinant Antibody (clone GAL-F2) | ELISA, FuncS, WB | Mouse IgG |
| MRO-0576-CN | Rabbit Anti-FGF2 Recombinant Antibody (clone JM28-10) | WB, IP, FC | Rabbit IgG |
| ZG-0343J | Mouse Anti-FGF2 Recombinant Antibody (ZG-0343J) | WB, IHC, ELISA | Mouse IgG |
| VS3-XY701 | Mouse Anti-FGF2 Recombinant Antibody (clone 2H5G2C11) | ELISA, WB, IHC |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-800CQ | Mouse Anti-FGF2 Recombinant Antibody (clone 0.T.50) | Neut | Mouse IgG1, κ |
| NEUT-801CQ | Mouse Anti-FGF2 Recombinant Antibody (clone CBL979) | Neut | Mouse IgG1, κ |
| NEUT-802CQ | Recombinant Mouse Anti-FGF2 Antibody (FB-8) | RIA, ELISA, Dot, Neut, WB, IHC-FoFr | IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1293 | Hi-Affi™ Rabbit Anti-FGF2 Recombinant Antibody (clone DS1293AB) | FC, IP, WB | Rabbit IgG |
Neuroscience Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-009CN | Human Anti-FGF2 Recombinant Antibody (clone YC055) | ELISA | Humanized IgG |
| NS-026CN | Mouse Anti-FGF2 Recombinant Antibody (clone Dan-2) | ELISA, FC | Mouse IgG |
| NS-009CN-F(E) | Human Anti-FGF2 Recombinant Antibody (clone YC056); Fab Fragment | ELISA | Humanized Fab |
| NS-026CN-F(E) | Mouse Anti-FGF2 Recombinant Antibody (clone YC058); Fab Fragment | ELISA, FC | Mouse Fab |
| NS-009CN-S(P) | Human Anti-FGF2 Recombinant Antibody (clone YC054); scFv Fragment | ELISA | Humanized scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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