Mouse Anti-FGF2 Recombinant Antibody (clone E12)
CAT#: HPAB-0164-WJ
This product is a mouse antibody targeting human basic fibroblast growth factor (bFGF/FGF2). This antibody is capable of fibroblast growth factor receptor competition binding human basic fibroblast cell growth factor (bFGF), can block the bFGF and tumor cell surface expression of the FGFR binding, blocking bFGF / FGFR pathway of signal transduction, and thus can effectively inhibit tumor cell proliferation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/641_G7_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human caudate
Immunohistochemical staining of human caudate shows nuclear positivity in glia.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/65502/1336_684_200x200.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/640_G7_1_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/642_G7_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_9_5.jpg
Normal Tissue
Figure 6 Caudate
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_8_7.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_8_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_9_5.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, fHDF/TERT166, hTERT-HME1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138685-FGF2
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Specifications
- Immunogen
- Recombinant human basic fibroblast growth factor (bFGF)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human FGF2
- Species Reactivity
- Human
- Clone
- E12
- Applications
- ELISA
- Related Disease
- Lung cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The FGF2 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Inhibition.
For ELISA: Recombinant human bFGF (50 ng/well) was used to coat microtiter ELISA wells. The ELISA wells were blocked with 5% non-fat dry milk in PBS buffer containing 0.5% Tween-20 for 1 h at 37°C, bFGF incubated with purified antibody (purified with Protein G Sepharose, 1:400,000) at 37°C for 1 h, then HRP-conjugated goat anti-mouse antibody (1:8,000) at 37°C for 40 min. The absorbance values were measured at 450 µm on an automatic plate reader.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone E12"
- CAT
- Product Name
See other products for "FGF2"
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|---|---|---|---|
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| HPAB-0164-WJ-F(E) | Mouse Anti-FGF2 Recombinant Antibody (clone E12); Fab Fragment | ELISA | Mouse Fab |
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