What is the immunogen used for this FGFR1 antibody?

This Rabbit Anti-FGFR1 Recombinant Antibody (clone CBACN-225) was generated using a recombinant protein corresponding to Human FGFR1 amino acids 360-375. It binds specifically to Human FGFR1 and has been validated for use in Western Blot (WB) and Immunofluorescence (IF).

How is this antibody purified and what is the buffer composition?

The antibody is purified using Protein A affinity chromatography, achieving greater than 95% purity by SDS-PAGE. It is supplied in TBS, pH 7.4, containing 1% BSA, 40% Glycerol, and 0.05% Sodium azide. The glycerol content aids in stability during storage at -20°C.

Rabbit Anti-FGFR1 Recombinant Antibody (clone CBACN-225)

CAT#: MRO-0578-CN

This antibody is a rabbit monoclonal antibody that is capable of binding to FGFR1.

Gene Expression

Normal Tissue Figure 1 Cerebral cortex

Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33614/67230_B_9_5.jpg

Normal Tissue Figure 2 Colon

Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33614/67230_A_9_3.jpg

Normal Tissue Figure 3 Kidney

Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33614/67230_A_8_5.jpg

Normal Tissue Figure 4 Testis

Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33614/67230_A_6_6.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (AN3-CA, BJ hTERT+)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000077782-FGFR1

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Specifications

Immunogen
Recombinant protein within Human FGFR1 aa 360-375

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human FGFR1

Species Reactivity
Human

Clone
CBACN-225

Applications
WB, IF

Conjugate
Unconjugated

Product Property

Purification
Purified with Protein A affinity chromatography

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
TBS, pH 7.4, 1% BSA, 40% Glycerol

Preservative
0.05% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Fibroblast Growth Factor Receptor 1; Basic Fibroblast Growth Factor Receptor 1; Fms-Related Tyrosine Kinase 2; Proto-Oncogene C-Fgr; EC 2.7.10.1; BFGF-R-1; FGFR-1; N-SAM; BFGFR; FGFBR; FLT-2; HBGFR; FLT2; CEK; FLG;

Gene ID
2260

UniProt ID
P11362

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Breast cancer biomarkers at key points during disease progression

Clear Immunofluorescence Imaging

We used this Rabbit Anti-FGFR1 Recombinant Antibody for immunofluorescence staining of cell lines. The clone CBACN-225 provided distinct and bright staining of the FGFR1 protein. It binds well to the specific amino acid region 360-375, allowing us to visualize the receptor distribution accurately without significant background.

Robust Western Blot Bands

Finding a reliable antibody for FGFR1 in Western Blot can be challenging, but this rabbit monoclonal antibody worked perfectly. We observed clean bands at the expected molecular weight. The Protein A affinity purification really shows in the quality of the blot, making data interpretation much more straightforward for our team.

Q&As

What is the immunogen used for this FGFR1 antibody?

A: This Rabbit Anti-FGFR1 Recombinant Antibody (clone CBACN-225) was generated using a recombinant protein corresponding to Human FGFR1 amino acids 360-375. It binds specifically to Human FGFR1 and has been validated for use in Western Blot (WB) and Immunofluorescence (IF).
How is this antibody purified and what is the buffer composition?

A: The antibody is purified using Protein A affinity chromatography, achieving greater than 95% purity by SDS-PAGE. It is supplied in TBS, pH 7.4, containing 1% BSA, 40% Glycerol, and 0.05% Sodium azide. The glycerol content aids in stability during storage at -20°C.

View the frequently asked questions answered by Creative Biolabs Support.

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Related Diseases

EGFR Tyrosine Kinase Inhibitor Resistance

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MRO-0578-CN.pdf

MSDS

MSDS - MRO-0578-CN.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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