Can antibodies be employed in serum-free media?

Some antibodies may perform better in serum than in serum-free media. Test your antibody under specific assay conditions to determine suitability.

Which solution works best for diluting my antibody?

Typically, manufacturers recommend buffers such as TBS or PBS with additional stabilizers like BSA for diluting antibodies.

How do I improve signal detection in my assays?

To enhance signal detection, optimize antigen retrieval, antibody concentration, incubation times, and detection methods.

Afuco™ Anti-FOLH1 ADCC Recombinant Antibodyc, ADCC Enhanced (AFC-TAB-018)

CAT#: AFC-TAB-018

This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to Human PMSA. It is a mouse monoclonal antibody which recognizes prostate specific membrane antigen (PSMA) from prostate cancer cells and normal prostate tissue.

Gene Expression

Normal Tissue Figure 1 Kidney

Proximal tubules (microvilli)
Staining: High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1451/4354_A_7_5.jpg

Normal Tissue Figure 2 Prostate

Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1451/4354_A_2_5.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enhanced (HAP1, RPMI-8226, SK-BR-3, TIME, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000086205-FOLH1

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Mouse

Derivation
Mouse

Type
ADCC enhanced antibody

Antibody Isotype
IgG1, κ

Species Reactivity
Human

Applications
ELISA, FC, IP, FuncS, IF, Neut

Conjugate
Unconjugated

Trade name
Prostascint

Generic Name
Capromab

Product Property

Purity
>95% by SDS-PAGE

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
FOLH1; folate hydrolase (prostate-specific membrane antigen) 1; FOLH; glutamate carboxypeptidase 2; GCP2; GCPII; glutamate carboxylase II;

Gene ID
2346

UniProt ID
Q04609

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Breast cancer biomarkers at key points during disease progression

High Binding Efficiency

In our experiments, robust binding efficiency ensures unambiguous and conclusive results. Our faith in the validity of our results has increased.

Reliability under Stress

During sample preparation, the antibody has shown to be dependable, retaining its effectiveness and yielding consistent results even under pressure.

Congenial Technical Support

The technical support was excellent in helping us optimize the antibody's potential in our particular experimental environment by guiding us through the optimization steps.

Q&As

Can antibodies be employed in serum-free media?

A: Some antibodies may perform better in serum than in serum-free media. Test your antibody under specific assay conditions to determine suitability.
Which solution works best for diluting my antibody?

A: Typically, manufacturers recommend buffers such as TBS or PBS with additional stabilizers like BSA for diluting antibodies.
How do I improve signal detection in my assays?

A: To enhance signal detection, optimize antigen retrieval, antibody concentration, incubation times, and detection methods.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AFC-TAB-018.pdf

MSDS

MSDS - AFC-TAB-018.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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