Human Anti-GPC3 Recombinant Antibody (clone hYP9.1) (CAT#: FAMAB-0560WJ)
This product is a humanized monoclonal antibody that is specific for human GPC3. This antibody can be used in a variety of applications, such as ELISA, WB.
Specific Inquiry
Figure 1 The affinity and cytotoxicity measurement of anti-GPC3 immunotoxins.
The binding of immunotoxin on GPC3+ cells.
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
Figure 2 The affinity and cytotoxicity measurement of anti-GPC3 immunotoxins.
The cytotoxicity of immunotoxins on cells. IT, immunotoxin.
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
FC
Figure 3 Binding of hYP7 and hYP9.1b (all in IgG format) on cells.
A431 cells are GPC3 negative.
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
CDC
Figure 4 CDC assays of anti-GPC3 antibodies.
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
ADCC
Figure 5 ADCC assays with various antibody concentrations and at an effector:target cell ratio of 25:1.
The luminescence from the wells without antibodies was treated as background and subtracted in panels (b,d). The luminescence from the wells with control antibodies at indicated concentrations was treated as background and subtracted. A431 cell is GPC3- and G1 cell is A431 cell overexpressing GPC3. Asterisk (*) indicate statistical significance (p < 0.05, tested by t test).
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
Specifications
- Immunogen
- A C-terminal peptide derived from human GPC3 isoform 2 (Residues: 511-560)
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human IgG1, κ
- Specificity
- Human GPC3
- Species Reactivity
- Human
- Clone
- hYP9.1
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-069 | Recombinant Anti-Mouse Gpc3 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
| TAB-416CQ | Anti-Human GPC3 Single Domain Antibody (TAB-416CQ), Research Grade Biosimilar | IP, IHC, FuncS, WB, ELISA, Inhib | Single domain antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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