Mouse Anti-GPC3 Recombinant Antibody (clone YP8) (CAT#: FAMAB-0556WJ)
This product is a mouse monoclonal antibody that is specific for human GPC3. This antibody can be used in a variety of applications, such as ELISA, WB.
Specific Inquiry
Figure 1 Binding of immunotoxins on G1 cells.
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
Figure 2 Cytotoxicity of immunotoxins on G1 cells. IT, immunotoxin.
The numbers in parentheses are EC50 values of immunotoxins in ng/ml.
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
FC
Figure 3 Flow cytometry analysis of five selected mouse hybridoma clones (YP6, YP7, YP8, YP9 and YP9.1) (solid lines) and 1G12, a control anti-GPC3 mAb (dotted lines, in the middle) using A431/G1 and Hep3B.
Filled peak: isotype control.
Phung, Y., Gao, W., Man, Y. G., Nagata, S., & Ho, M. (2012, September). High-affinity monoclonal antibodies to cell surface tumor antigen glypican-3 generated through a combination of peptide immunization and flow cytometry screening. In MAbs (Vol. 4, No. 5, pp. 592-599). Taylor & Francis.
ELISA
Figure 4 ELISA analysis of five hybridoma clones on recombinant GPC3 proteins.
GPC3: recombinant GPC3-human Fc; GPC3ΔHS: recombinant GPC3 without HS; control: BSA. 1G12: a commercial anti-GPC3 mAb.
Phung, Y., Gao, W., Man, Y. G., Nagata, S., & Ho, M. (2012, September). High-affinity monoclonal antibodies to cell surface tumor antigen glypican-3 generated through a combination of peptide immunization and flow cytometry screening. In MAbs (Vol. 4, No. 5, pp. 592-599). Taylor & Francis.
Specifications
- Immunogen
- A C-terminal peptide derived from human GPC3 isoform 2 (Residues: 511-560)
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgG1, κ
- Specificity
- Human GPC3
- Species Reactivity
- Human
- Clone
- YP8
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "GPC3"
Select a product category from the dropdown menu below to view related products.
Please select product type
Single-domain Antibody
Immunotoxin
Human Antibody
Chimeric Antibody
Humanized Antibody
Mouse Antibody
MHC Tetramer for Cancer
Rabbit Monoclonal Antibody
Recombinant Antibody
Fab Fragment Antibody
ADCC Enhanced Antibody
scFv Fragment Antibody
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-069 | Recombinant Anti-Mouse Gpc3 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
| TAB-416CQ | Anti-Human GPC3 Single Domain Antibody (TAB-416CQ), Research Grade Biosimilar | IP, IHC, FuncS, WB, ELISA, Inhib | Single domain antibody |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for FAMAB-0556WJ. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: FC, FuncS, IHC, ELISA
-1-1.png)
Application: ELISA
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, Inhib, FuncS
Application: ELISA, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
