Mouse Anti-GPC3 Recombinant Antibody (clone YP9) (CAT#: FAMAB-0557WJ)
This product is a mouse monoclonal antibody that is specific for human GPC3. This antibody can be used in a variety of applications, such as ELISA, WB.
Specific Inquiry
FC
Figure 1 Flow cytometry analysis of five selected mouse hybridoma clones (YP6, YP7, YP8, YP9 and YP9.1) (solid lines) and 1G12, a control anti-GPC3 mAb (dotted lines, in the middle) using A431/G1 and Hep3B.
Filled peak: isotype control.
Phung, Y., Gao, W., Man, Y. G., Nagata, S., & Ho, M. (2012, September). High-affinity monoclonal antibodies to cell surface tumor antigen glypican-3 generated through a combination of peptide immunization and flow cytometry screening. In MAbs (Vol. 4, No. 5, pp. 592-599). Taylor & Francis.
ELISA
Figure 2 ELISA analysis of five hybridoma clones on recombinant GPC3 proteins.
GPC3: recombinant GPC3-human Fc; GPC3ΔHS: recombinant GPC3 without HS; control: BSA. 1G12: a commercial anti-GPC3 mAb.
Phung, Y., Gao, W., Man, Y. G., Nagata, S., & Ho, M. (2012, September). High-affinity monoclonal antibodies to cell surface tumor antigen glypican-3 generated through a combination of peptide immunization and flow cytometry screening. In MAbs (Vol. 4, No. 5, pp. 592-599). Taylor & Francis.
WB
Figure 3 western blot analysis of YP7 and YP9.
Thirty μg of whole cell lysates of HepG2, Hep3B, A431/G1 (A431.GPC3+) and A431 was loaded and probed with each hybridoma supernatant.
Phung, Y., Gao, W., Man, Y. G., Nagata, S., & Ho, M. (2012, September). High-affinity monoclonal antibodies to cell surface tumor antigen glypican-3 generated through a combination of peptide immunization and flow cytometry screening. In MAbs (Vol. 4, No. 5, pp. 592-599). Taylor & Francis.
Figure 4 Binding of immunotoxins on G1 cells.
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
Figure 5 Cytotoxicity of immunotoxins on G1 cells. IT, immunotoxin.
The numbers in parentheses are EC50 values of immunotoxins in ng/ml.
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
Specifications
- Immunogen
- A C-terminal peptide derived from human GPC3 isoform 2 (Residues: 511-560)
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgG1, κ
- Specificity
- Human GPC3
- Species Reactivity
- Human
- Clone
- YP9
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1563z | Mouse Anti-GPC3 Recombinant Antibody (clone 29F5) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| VS3-WK133 | Mouse Anti-GPC3 Recombinant Antibody (clone G3-C2) | WB, ELISA | Mouse IgG1 |
| VS3-FY605 | Recombinant Mouse Anti-GPC3 Antibody (clone 2A2C3) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY606 | Recombinant Mouse Anti-GPC3 Antibody (clone 2A2H1) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY607 | Recombinant Mouse Anti-GPC3 Antibody (clone 2C7E9) | ELISA, WB, IHC, FC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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