Mouse Anti-HIF1A Monoclonal Antibody (VS7-0425-WR34) (CAT#: VS7-0425-WR34)
This product is a mouse IgG antibody that binds to human HIF1A. The antibody can be used for WB, IHC-P, IF, ICC, and ELISA assays. It can cross reacts with mouse and monkey.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Normal Tissue
RNA Expression
Figure 1 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/ihc_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17442/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17442/ihc_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_B_8_5.jpg
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_B_8_5.jpg
Figure 4 Gallbladder
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_5_4.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_5_4.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_8_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (LHCN-M2)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100644-HIF1A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100644-HIF1A
Specifications
- Immunogen
- Recombinant human HIF1A fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HIF1A
- Species Reactivity
- Human, Mouse, Monkey
- Molecule Class
- Transcription Factor
- Applications
- WB, IHC-P, IF, ICC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Cancer; Atherosclerosis; Chronic obstructive pulmonary disease (COPD); Pulmonary hypertension; Diabetic complications; Inflammatory diseases; Preeclampsia
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/mL
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Immunohistochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
- Long Name
- Hypoxia inducible factor 1 subunit alpha
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Glycoprotein; Hydroxylation; Isopeptide bond; Phosphoprotein; S-nitrosylation; Ubl conjugation
- Protein Refseq
- NP_003484
- Function
- Activator; DNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "HIF1A"
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Mouse Antibody Products
IgG Antibody Products
Rabbit Monoclonal Antibody Products
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-080CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (6A9) | WB, Inhibit | |
| TAB-081CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (1H1) | WB, Inhibit | |
| TAB-082CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (6H4) | WB, Inhibit | |
| TAB-083CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (5A5) | WB, Inhibit | |
| TAB-084CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (7D6) | WB, Inhibit |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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