Mouse Anti-HIF1A Monoclonal Antibody (VS7-0425-WR34)
CAT#: VS7-0425-WR34
This product is a mouse IgG antibody that binds to human HIF1A. The antibody can be used for WB, IHC-P, IF, ICC, and ELISA assays. It can cross reacts with mouse and monkey.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17442/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_B_8_5.jpg
Normal Tissue
Figure 4 Gallbladder
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_5_4.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (LHCN-M2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100644-HIF1A
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Specifications
- Immunogen
- Recombinant human HIF1A fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HIF1A
- Species Reactivity
- Human, Mouse, Monkey
- Molecule Class
- Transcription Factor
- Applications
- WB, IHC-P, IF, ICC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Cancer; Atherosclerosis; Chronic obstructive pulmonary disease (COPD); Pulmonary hypertension; Diabetic complications; Inflammatory diseases; Preeclampsia
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/mL
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Immunohistochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
- Long Name
- Hypoxia inducible factor 1 subunit alpha
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Glycoprotein; Hydroxylation; Isopeptide bond; Phosphoprotein; S-nitrosylation; Ubl conjugation
- Protein Refseq
- NP_003484
- Function
- Activator; DNA-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HIF1A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1828z | Mouse Anti-HIF1A Recombinant Antibody (clone 6F11) | WB, ELISA, IF | Mouse IgG1 |
| MOB-0903CT | Recombinant Mouse anti-Human HIF1A Monoclonal antibody (2B4) | ELISA, ICC, IF, IHC-P, WB | |
| MRO-1928-CN | Rabbit Anti-HIF1A Polyclonal Antibody (MRO-1928-CN) | WB, IF, FC | Rabbit IgG |
| VS3-QX510 | Mouse Anti-HIF1A Recombinant Antibody (clone 5H7) | WB, IP, ICC, IHC | Mouse IgG1 |
| VS3-WK1354 | Mouse Anti-HIF1A Recombinant Antibody (VS3-WK1354) | WB, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-080CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (6A9) | WB, Inhibit | |
| TAB-081CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (1H1) | WB, Inhibit | |
| TAB-082CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (6H4) | WB, Inhibit | |
| TAB-083CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (5A5) | WB, Inhibit | |
| TAB-084CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (7D6) | WB, Inhibit |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1595 | Rabbit Anti-HIF1A Recombinant Antibody (clone DS1595AB) | IHC, WB, IHC-P | Rabbit IgG |
| MOR-4589 | Rabbit Anti-HIF1A Recombinant Antibody (clone TH102DS) | WB, IF, ICC, FC, ChIP, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY248 | Mouse Anti-NHP HIF1A Recombinant Antibody (clone H1alpha67) | WB, IHC, IP, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3136 | Anti-HIF1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3137 | Anti-Mouse HIF1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3138 | Anti-Porcine HIF1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3139 | Anti-Rabbit HIF1A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC156 | SmartAb™ Recombinant Anti-HIF1A pH-dependent Antibody (Clone 1H1) | WB | Mouse IgG2a kappa |
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