Rabbit Anti-HIF1A Recombinant Antibody (clone 5D12) (CAT#: ZG-0038U)
The purified Rabbit Anti-HIF1A Recombinant Antibody (clone 5D12) displayed high specificity against the HIF1A protein of human.
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IF
Figure 1 Rabbit Anti-HIF1A Antibody (ZG-0038U) in IF
Immunofluorescence staining of Hela Cells with ZG-0038U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human HIF-1 alpha
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HIF1A
- Species Reactivity
- Human
- Clone
- 5D12
- Applications
- ELISA, IF
- Conjugate
- Unconjugated
- Related Disease
- Hypoxia and Retinal Ischemia
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunofluorescence. Specific conditions should be optimized by the end user.
IF:1:20-1:200
Target
- Alternative Names
- Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8; HIF1A; BHLHE78; MOP1; PASD8
- Gene ID
- 3091
- UniProt ID
- Q16665
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Mouse Antibody Products
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-080CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (6A9) | WB, Inhibit | |
| TAB-081CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (1H1) | WB, Inhibit | |
| TAB-082CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (6H4) | WB, Inhibit | |
| TAB-083CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (5A5) | WB, Inhibit | |
| TAB-084CT-S(P) | Anti-Human HIF-1alpha Recombinant Antibody scFv Fragment (7D6) | WB, Inhibit |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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