Mouse Anti-HIF1A Recombinant Antibody (clone 5H7)
CAT#: VS3-QX510
This product is a mouse antibody that recognizes HIF1A. The antibody can be used in applications: WB, IP, ICC, IHC.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17442/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_B_8_5.jpg
Normal Tissue
Figure 4 Gallbladder
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_5_4.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (LHCN-M2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100644-HIF1A
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Specifications
- Immunogen
- Peptide
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- HIF-1 alpha
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 5H7
- Applications
- WB, IP, ICC, IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH7.4), 1% BSA, 40% Glycerol.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HIF1A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-080CT | Anti-Human HIF-1alpha Recombinant Antibody (6A9) | FuncS | |
| TAB-081CT | Anti-Human HIF-1alpha Recombinant Antibody (1H1) | WB | |
| TAB-082CT | Anti-Human HIF-1alpha Recombinant Antibody (6H4) | WB | |
| TAB-083CT | Anti-Human HIF-1alpha Recombinant Antibody (5A5) | WB, Inhibit | |
| TAB-084CT | Anti-Human HIF-1alpha Recombinant Antibody (7D6) | WB, Inhibit |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1595 | Rabbit Anti-HIF1A Recombinant Antibody (clone DS1595AB) | IHC, WB, IHC-P | Rabbit IgG |
| MOR-4589 | Rabbit Anti-HIF1A Recombinant Antibody (clone TH102DS) | WB, IF, ICC, FC, ChIP, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1928-CN | Rabbit Anti-HIF1A Polyclonal Antibody (MRO-1928-CN) | WB, IF, FC | Rabbit IgG |
| MRO-1929-CN | Rabbit Anti-HIF1A Polyclonal Antibody (MRO-1929-CN) | IF, IHC, FC | Rabbit IgG |
| ZG-0038U | Rabbit Anti-HIF1A Recombinant Antibody (clone 5D12) | ELISA, IF | Rabbit IgG |
| VS3-XY804 | Mouse Anti-HIF1A Recombinant Antibody (clone 5B1) | IHC | |
| VS3-XY805 | Mouse Anti-HIF1A Recombinant Antibody (clone 1A3) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY248 | Mouse Anti-NHP HIF1A Recombinant Antibody (clone H1alpha67) | WB, IHC, IP, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3136 | Anti-HIF1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3137 | Anti-Mouse HIF1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3138 | Anti-Porcine HIF1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3139 | Anti-Rabbit HIF1A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC156 | SmartAb™ Recombinant Anti-HIF1A pH-dependent Antibody (Clone 1H1) | WB | Mouse IgG2a kappa |
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