Mouse Anti-ICAM1 Recombinant Antibody (clone PT0050) (CAT#: VS3-CJ409)
This product is a mouse antibody that recognizes ICAM1.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/29_G3_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/29_G3_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2142/203_G3_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2142/203_G3_1_selected.jpg
Figure 3 IF staining of human cell line EFO-21
(Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/1937_A10_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/1937_A10_1_blue_red_green.jpg
Figure 4 IF staining of human cell line GAMG
(Immunofluorescent staining of human cell line GAMG shows localization to plasma membrane, vesicles, nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/2058_A10_4_blue_red_green.jpg
(Immunofluorescent staining of human cell line GAMG shows localization to plasma membrane, vesicles, nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/2058_A10_4_blue_red_green.jpg
Figure 5 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_B_9_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_B_9_5.jpg
Figure 6 Lung
(Alveolar cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_A_3_4.jpg
(Alveolar cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_A_3_4.jpg
Figure 7 Colon
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_3.jpg
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_3.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_5.jpg
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_5.jpg
Figure 9 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_9_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_9_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (HHSteC, Karpas-707, RPMI-8226)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000090339-ICAM1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000090339-ICAM1
Specifications
- Immunogen
- Synthesized peptide derived from human CD54
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ICAM1
- Species Reactivity
- Human
- Clone
- PT0050
- Applications
- WB, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:2000), Immunohistochemistry-Parraffin (1:100-1:500).
Target
- Alternative Names
- Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD antigen CD54; BB2; CD54; P3.58
- Gene ID
- 3383
- UniProt ID
- P05362
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. ICAM family.
- Cellular Localization
- Membrane
- Post Translation Modifications
- Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
- Protein Refseq
- NP_000192.2
- Function
- ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
(Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins.
(Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ICAM1"
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1937-VHH | Recombinant Anti-human ICAM1 VHH Single Domain Antibody | FA, FC, ICC, ELISA | Llama VHH |
| TAB-126CT | Llama Anti-ICAM1 Recombinant Antibody (TAB-126CT) | ELISA, IF | Llama VHH |
| HPAB-0349-YC-VHH | Recombinant Llama Anti-ICAM1 Single Domain Antibody (34-1) | ELISA | Llama VHH |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1136 | Human Anti-ICAM1 Recombinant Antibody (clone YC170); scFv Fragment | ELISA, WB, IHC, IP | Human scFv |
| DrMAB-1139 | Human Anti-ICAM1 Recombinant Antibody (clone YC171); scFv Fragment, HRP | ELISA, WB, IHC, IP | Human scFv |
| PSBZ-079 | Mouse Anti-ICAM1 Recombinant Antibody (clone CA7); scFv Fragment | FC | Mouse scFv |
| HPAB-0053-YC-S(P) | Human Anti-ICAM1 Recombinant Antibody (clone B11); scFv Fragment | FC, IP | Human scFv |
| HPAB-0043-FY-S(P) | Human Anti-ICAM1 Recombinant Antibody (clone Icmo0191); scFv Fragment | ELISA | Human scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-241 | Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (Enlimomab Pegol) | WB, IF, IP, Neut, FuncS, ELISA, FC | Fab' - G2a |
| TAB-H26 | Anti-Human ICAM1 Recombinant Antibody (Enlimomab) | Neut, ELISA, IF, IP, FuncS, FC, WB | IgG2a |
| TAB-124CT | Anti-Human ICAM-1 Recombinant Antibody (m1A6) | ELISA | |
| TAB-124CT-S(P) | Anti-Human ICAM-1 Recombinant Antibody scFv Fragment (m1A6) | ELISA | |
| TAB-124CT-F(E) | Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (m1A6) | ELISA |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L023R | anti-ICAM1 immunotoxin UV3 (IgG)-RTA | Cytotoxicity assay, Functional assay |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-123CT | Anti-Human ICAM-1 Recombinant Antibody (h1A6) | ELISA | Humanized antibody |
| TAB-123CT-S(P) | Anti-Human ICAM-1 Recombinant Antibody scFv Fragment (h1A6) | ELISA | Humanized antibody |
| TAB-123CT-F(E) | Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (h1A6) | ELISA | Humanized antibody |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-121CT-S(P) | Anti-Human ICAM-1 Recombinant Antibody scFv Fragment (MD-2) | ELISA, FC | Human antibody |
| TAB-122CT-S(P) | Anti-Human ICAM-1 Recombinant Antibody scFv Fragment (R6-5-D6) | ELISA, FC | Human antibody |
| TAB-125CT-S(P) | Anti-Human ICAM-1 Recombinant Antibody scFv Fragment (ICM 10064) | Inhibit, ELISA, WB | Human antibody |
| TAB-121CT-F(E) | Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (MD-2) | ELISA, FC | Human antibody |
| TAB-125CT-F(E) | Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (ICM 10064) | Inhibit, ELISA, WB | Human antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-104LC | Recombinant Anti-Human ICAM1 Antibody (Fc glycosylation) | ELISA | Mouse antibody |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-923CQ | Mouse Anti-ICAM1 Recombinant Antibody (clone CBL236) | Neut, IF, FC, WB, IHC | Mouse IgG1 |
| NEUT-925CQ | Mouse Anti-ICAM1 Recombinant Antibody (clone HA58) | FC, FuncS, IHC, Neut, WB | Mouse IgG1, κ |
| NEUT-926CQ | Mouse Anti-ICAM1 Recombinant Antibody (VMC182) | FC, IF, IHC, ICC, Neut | Mouse IgG2b |
| NEUT-927CQ | Mouse Anti-ICAM1 Recombinant Antibody (clone RR1/1) | FC, IF, IHC, Neut, WB, ELISA | Mouse IgG1 |
| NEUT-933CQ | Mouse Anti-Icam1 Recombinant Antibody (clone 1A29) | WB, IP, ICC, IF, IHC-P, Neut, FC | Mouse IgG1 |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-924CQ | Mouse Anti-ICAM1 Recombinant Antibody (clone HCD54) | FC, IF, Block | Mouse IgG1, κ |
| NEUT-928CQ | Mouse Anti-ICAM1 Recombinant Antibody (VMC183) | Block, FC, IHC, IP | Mouse IgG1 |
| NEUT-929CQ | Mouse Anti-ICAM1 Recombinant Antibody (clone BBIG-I1(11C81)) | Block, WB, IP, ICC | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1723 | Rabbit Anti-ICAM1 Recombinant Antibody (clone DS1723AB), Biotin | IHC-Fr, IP, WB, ELISA | Rabbit IgG |
| MOR-1724 | Rabbit Anti-Icam1 Recombinant Antibody (clone DS1724AB) | ELISA | Rabbit IgG |
| MOR-4598 | Rabbit Anti-ICAM1 Recombinant Antibody (clone TH111DS) | WB, FC | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0053-YC | Human Anti-ICAM1 Recombinant Antibody (clone B11) | Inhib, FC, IHC | Human IgG1 |
| MRO-0805-CN | Mouse Anti-ICAM1 Recombinant Antibody (clone F11-A4) | WB, IF, IHC | Mouse IgG1a |
| MRO-0806-CN | Mouse Anti-ICAM1 Recombinant Antibody (clone J0-F7) | WB, IF, IHC, FC | Mouse IgG2a |
| MRO-0807-CN | Rabbit Anti-ICAM1 Recombinant Antibody (clone CBACN-298) | WB, IF, IHC | Rabbit IgG |
| MRO-1952-CN | Rabbit Anti-ICAM1 Polyclonal Antibody (MRO-1952-CN) | WB, IF, IHC, FC | Rabbit IgG |
Epitope-Specific Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-1635LC | Mouse Anti-ICAM1 Recombinant Antibody (clone c78.4A) | FC | Mouse IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0043-FY-F(E) | Human Anti-ICAM1 Recombinant Antibody (clone Icmo0191); Fab Fragment | ELISA | Human Fab, λ |
| HPAB-0859-CN-F(E) | Human Anti-ICAM1 Recombinant Antibody (clone BI-AB); Fab Fragment | ELISA, FC, Neut | Human Fab |
| HPAB-S0062-YC-F(E) | Human Anti-ICAM1 Recombinant Antibody (clone HumB); Fab Fragment | ELISA, FuncS | Humanized Fab |
| HPAB-S0063-YC-F(E) | Human Anti-ICAM1 Recombinant Antibody (clone HumC); Fab Fragment | ELISA, FuncS | Humanized Fab |
| HPAB-S0064-YC-F(E) | Human Anti-ICAM1 Recombinant Antibody (clone HumD); Fab Fragment | ELISA, FuncS | Humanized Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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