Mouse Anti-ICAM1 Recombinant Antibody (VS4-WK235)

Name: Mouse Anti-ICAM1 Recombinant Antibody (VS4-WK235)
SKU: VS4-WK235
Rating: 5 (1 reviews)

CAT#: VS4-WK235

This product is a mouse monoclonal antibody that recognizes ICAM1 and can be used in ELISA, WB, ICC.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/29_G3_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2142/203_G3_1_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line EFO-21

Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/1937_A10_1_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line GAMG

Immunofluorescent staining of human cell line GAMG shows localization to plasma membrane, vesicles, nucleoplasm & centrosome.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/2058_A10_4_blue_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_B_9_5.jpg

Normal Tissue Figure 6 Lung

Alveolar cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_A_3_4.jpg

Normal Tissue Figure 7 Colon

Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_3.jpg

Normal Tissue Figure 8 Kidney

Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_5.jpg

Normal Tissue Figure 9 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_9_8.jpg

RNA Expression Figure 10 RNA cell line category: Cell line enhanced (HHSteC, Karpas-707, RPMI-8226)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000090339-ICAM1

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Specifications

Immunogen
Purified recombinant human ICAM1 (C-terminus) protein fragments expressed in E.coli.

Host Species
Mouse

Derivation
Mouse

Type
Mouse IgG

Specificity
Human ICAM1

Species Reactivity
Human

Applications
ELISA, WB, ICC

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS containing 50% glycerol, pH 7.4.

Preservative
0.02% sodium azide

Storage
Store at -20°C or -80°C. Avoid repeated freeze.

Shipping
Ice packs

Applications

Application Notes
Recommended dilution: WB:1:500-1:5000, ICC:1:50-1:200

Target

Alternative Names
Intercellular Adhesion Molecule 1

Gene ID
3383

UniProt ID
P05362

Long Name
Intercellular Adhesion Molecule 1

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Related Signaling Pathways

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS4-WK235.pdf

MSDS

MSDS - VS4-WK235.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Isotype Control

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Secondary Antibody

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