Mouse Anti-IgE Recombinant Antibody (clone MaE11) (CAT#: HPAB-AP678-YC)

Figure 1 Effects of anti-IgE mAbs on spontaneous and ragweed-specific histamine release.

Histamine release was performed as previously published, Inhibition of ragweed-induced histamine release was measured by coincubating anti-IgE mAbs with lOOng/ml ragweedspecific
IgE for 2 h at 37°C followed by a 30-min challenge with 100 ng/ml ragweed antigen. Data represents I of 3 experiments.

Shields, R. L., Werther, W. R., Zioncheck, K., O’Connell, L., Klassen, T., Fendly, B., ... & Jardieu, P. M. (1995). Anti-lgE monoclonal antibodies that inhibit allergen-specific histamine release. International archives of allergy and immunology, 107(1-3), 412-413.

Figure 2 Binding of recombinant FcεRI α subunit on CUO3DI0 cells and anti-IgE mAbs (Mae-11, -13 and -17) to recombinant C,3 domain mutants.

Mutants were prepared [4], transiently expressed and quantified by ELISA. Binding of CHO cells to 1 ug/ml IgE mutants was measured by immunofluorescence and analyzed by flow cytometry, and binding to anti-IgE mAbs was measured by ELISA. Values are the average of duplicate determinations from 1 of 3 experiments.

Shields, R. L., Werther, W. R., Zioncheck, K., O’Connell, L., Klassen, T., Fendly, B., ... & Jardieu, P. M. (1995). Anti-lgE monoclonal antibodies that inhibit allergen-specific histamine release. International archives of allergy and immunology, 107(1-3), 412-413.

Figure 3 Hllmanized MaEll mAb blocks AgE-mediated contraction and histamine release from passively sensitized human lung parenchyma.

Human lung strips were incubated with a 1 : 10 dilution of human ragweed serum for 20 hours in the presence of an equimolar concentration of MaE11 or a IO-fold excess concentration of control anti-IgE mAb, MaEl. The lo-fold excess concentration of MaEl inhibited histamine release (A) and tissue contraction (B) in response to AgE challenge by about 50%. In contrast, equimolar concentrations of MaEll completely blocked both histamine release (A) and tissue contraction (B) in response to AgE challenge. Results are expressed as the mean ± SEM of five separate experiments.

Saban, R., Haak-Frendscho, M., Zine, M., Ridgway, J., Gorman, C., Presta, L. G., ... & Jardieu, P. (1994). Human FcERI-IgG and humanized anti-IgE monoclonal antibody MaE11 block passive sensitization of human and rhesus monkey lung. Journal of allergy and clinical immunology, 94(5), 836-843.