Mouse Anti-IGFBP7 Recombinant Antibody (clone 1D9E7)
CAT#: VS7-0425-WR121
This product is a mouse IgG1 recombinant antibody that binds to human IGFBP7. The antibody 1D9E7 recognizes a specific amino acid domain of human IGFBP7 (AA: 52-156). It can be used for IHC assay.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20668/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20668/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Not detected
Intensity:Weak
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_A_8_4.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_A_5_6.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, hTERT-RPE1, RPTEC TERT1, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163453-IGFBP7
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Specifications
- Immunogen
- Recombinant human IGFBP7 (AA: 52-156) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human IGFBP7
- Species Reactivity
- Human
- Clone
- 1D9E7
- Molecule Class
- Insulin-Like Growth Factor-Binding Protein
- Applications
- IHC
- Conjugate
- Unconjugated
- Related Disease
- Retinal arterial macroaneurysm with supravalvular pulmonic stenosis (RAMSVPS)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
- Gene ID
- 3490
- UniProt ID
- Q16270
- Long Name
- Insulin Like Growth Factor Binding Protein 7
- Cellular Localization
- Secreted
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Phosphoprotein
- Protein Refseq
- NP_001240764.1
- Function
- Growth factor binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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