Mouse Anti-ITGA5 Monoclonal Antibody (VS7-0425-WR154)
CAT#: VS7-0425-WR154
This product is a mouse IgG antibody that binds to human ITGA5. The antibody can be used for WB, IHC-P, IF, FC, and ELISA assays.
Gene Expression
Normal Tissue
Figure 1 High expression in placenta
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/ihc_selected.jpg
Normal Tissue
Figure 2 Low expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_B_1_6_val_selected.jpg
Normal Tissue
Figure 3 IHC staining of human placenta
Immunohistochemical staining of human placenta shows distinct cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9008/ihc_selected.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_A_8_4.jpg
Normal Tissue
Figure 5 Placenta
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: Low Intensity: Moderate Quantity: <25% Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_A_3_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (hTERT-RPE1, HUVEC TERT2, TIME, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000161638-ITGA5
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Specifications
- Immunogen
- Recombinant human ITGA5 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ITGA5
- Species Reactivity
- Human
- Molecule Class
- Integrin/Cell Adhesion Molecule
- Applications
- WB, IHC-P, IF, FC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Various cancers; Fibrosis; Cardiovascular diseases
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- FNRA; CD49e; VLA-5; VLA5A
- Gene ID
- 3678
- UniProt ID
- P08648
- Long Name
- Integrin Subunit Alpha 5
- Cellular Localization
- Cell junction, Membrane
- Post Translation Modifications
- Proteolytic cleavage by PCSK5 mediates activation of the precursor.
- Protein Refseq
- NP_002196
- Function
- integrin binding; platelet-derived growth factor receptor binding; protein binding; receptor activity; vascular endothelial growth factor receptor 2 binding;
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ITGA5"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-077 | Anti-Human Integrin alpha 5 beta 1 Recombinant Antibody (Volociximab) | ELISA, IHC | IgG4 |
| CAT | Product Name | Application | Type |
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| NEUT-1554CQ | Recombinant Mouse Anti-ITGA5 Antibody (NKI-SAM-1) | FC, IP, ELISA, BL | IgG2b, κ |
| NEUT-1555CQ | Recombinant Armenian Hamster Anti-Itga5 Antibody (HMα5-1) | FC, BL, IP | IgG |
| NEUT-1556CQ | Recombinant Rat Anti-Itga5 Antibody (5H10-27) | FC, BL, IHC-Fr, FuncS | IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1874 | Hi-Affi™ Recombinant Rabbit Anti-ITGA5 Monoclonal Antibody (DS1874AB) | WB, IHC-P, ICC, IF, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0849-CN | Recombinant Rabbit Anti-ITGA5 Monoclonal Antibody (CBACN-310) | WB, IF, IHC, IP, FC | Rabbit IgG |
| HPAB-0398-WJ | Human Anti-ITGA5 Recombinant Antibody (HPAB-0398-WJ) | ELISA | Humanized IgG |
| HPAB-0399-WJ | Mouse Anti-ITGA5 Recombinant Antibody (HPAB-0399-WJ) | FC | Mouse IgG |
| FAMAB-1184CQ | Mouse Anti-ITGA5 Recombinant Antibody (clone SNAKA51) | IHC, WB, IF | Mouse IgG2a |
| VS3-FY770 | Recombinant Rabbit Anti-ITGA5 Antibody (clone R02-3G4) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-077 | Afuco™ Anti-ITGA5 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-077) | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0398-WJ-S(P) | Human Anti-ITGA5 Recombinant Antibody; scFv Fragment (HPAB-0398-WJ-S(P)) | ELISA | Humanized scFv |
| HPAB-0399-WJ-S(P) | Mouse Anti-ITGA5 Recombinant Antibody; scFv Fragment (HPAB-0399-WJ-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0398-WJ-F(E) | Human Anti-ITGA5 Recombinant Antibody; Fab Fragment (HPAB-0398-WJ-F(E)) | ELISA | Humanized Fab |
| HPAB-0399-WJ-F(E) | Mouse Anti-ITGA5 Recombinant Antibody; Fab Fragment (HPAB-0399-WJ-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1485 | AbPlus™ Anti-ITGA5 Magnetic Beads (VS-0724-YC1485) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1155 | Anti-ITGA5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3627 | Anti-Mouse ITGA5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3629 | Anti-Human ITGA5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3628 | Anti-Rat ITGA5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY136 | Rabbit Anti-ITGA5 scFv-Fc Chimera (VS-0425-FY136) | ELISA, Flow, ICC, IF, IHC, BLOCK | Rabbit IgG, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC570 | Recombinant Anti-ITGA5 Vesicular Antibody, EV Displayed (VS-0425-YC570) | ELISA, FC, Cell-uptake |
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