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    Mouse Anti-LGR5 Recombinant Antibody (clone 2B5B9) (CAT#: VS7-0425-WR285)

    This product is a mouse IgG2b antibody that binds to human LGR5. The antibody 2B5B9 recognizes specific amino acid domain of human LGR5 (AA: 22-178). It can be used for FC assay.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line Hep G2
    Figure 1 IF staining of human cell line Hep G2
    (Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/1404_H2_1_selected.jpg
    Figure 2 IHC staining of human colon
    Figure 2 IHC staining of human colon
    (Immunohistochemical staining of human colon shows cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/ihc_selected.jpg
    Figure 3 IF staining of human cell line A-431
    Figure 3 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/103_B2_1_blue_red_green.jpg
    Figure 4 IF staining of human cell line U-251 MG
    Figure 4 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to Golgi apparatus.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/102_B2_1_blue_red_green.jpg
    Normal Tissue
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glial cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear
    Neuropil
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_B_7_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_3.jpg
    Figure 7 Liver
    Figure 7 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_7_4.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cells in glomeruli
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_5.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_6_6.jpg
    RNA Expression
    Figure 10 RNA cell line category: Group enriched (Hep G2, SH-SY5Y)
    Figure 10 RNA cell line category: Group enriched (Hep G2, SH-SY5Y)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139292-LGR5

    Specifications

    • Immunogen
    • Recombinant human LGR5 (AA: 22-178) fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG2b
    • Specificity
    • Human LGR5
    • Species Reactivity
    • Human
    • Clone
    • 2B5B9
    • Molecule Class
    • G Protein-Coupled Receptor
    • Applications
    • FC
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Flow Cytometry: 1:200 - 1:400
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • LGR5; G Protein-Coupled Receptor 49; GRP49; G-Protein Coupled Receptor 67; Leucine-Rich Repeat-Containing G-Protein Coupled Receptor 5; G-Protein Coupled Receptor 49; GPR67; Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 5; Leucine-Rich Repeat
    • Long Name
    • Leucine Rich Repeat Containing G Protein-Coupled Receptor 5
    • Cellular Localization
    • Cell membrane; Golgi apparatus; Membrane
    • Post Translation Modifications
    • Disulfide bond; Glycoprotein
    • Function
    • G-protein coupled receptor; Receptor; Transducer

    Recommended Products

    Isotype Control

    Secondary Antibody

    Reconstitution Buffer

    Recommended Dilution Buffer

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "LGR5"

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    CAT Product Name Application Type
    NAB-1650-VHH Recombinant Anti-Human LGR5 VHH Single Domain Antibody WB, ICC, ChiP, FA, ELISA Llama VHH

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