Mouse Anti-LGR5 Recombinant Antibody (clone 2B5C3)

Name: Mouse Anti-LGR5 Recombinant Antibody (clone 2B5C3)
SKU: VS7-0425-WR286
Rating: 5 (1 reviews)

CAT#: VS7-0425-WR286

This product is a mouse IgG2b antibody that binds to human LGR5. The antibody 2B5C3 recognizes specific amino acid domain of human LGR5 (AA: 22-178). It can be used for WB and ELISA assays.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line Hep G2

Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/1404_H2_1_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human colon

Immunohistochemical staining of human colon shows cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/103_B2_1_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/102_B2_1_blue_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_B_7_5.jpg

Normal Tissue Figure 6 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_3.jpg

Normal Tissue Figure 7 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_7_4.jpg

Normal Tissue Figure 8 Kidney

Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_5.jpg

Normal Tissue Figure 9 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_6_6.jpg

RNA Expression Figure 10 RNA cell line category: Group enriched (Hep G2, SH-SY5Y)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139292-LGR5

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Specifications

Immunogen
Recombinant human LGR5 (AA: 22-178) fragment expressed in E. Coli.

Host Species
Mouse

Type
Mouse IgG2b

Specificity
Human LGR5

Species Reactivity
Human

Clone
2B5C3

Molecule Class
G Protein-Coupled Receptor

Applications
WB, ELISA

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
0.05% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000

Target

Alternative Names
LGR5; G Protein-Coupled Receptor 49; GRP49; G-Protein Coupled Receptor 67; Leucine-Rich Repeat-Containing G-Protein Coupled Receptor 5; G-Protein Coupled Receptor 49; GPR67; Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 5; Leucine-Rich Repeat

Gene ID
8549

UniProt ID
O75473

Long Name
Leucine Rich Repeat Containing G Protein-Coupled Receptor 5

Cellular Localization
Cell membrane; Golgi apparatus; Membrane

Post Translation Modifications
Disulfide bond; Glycoprotein

Protein Refseq
NP_001264155.1

Function
G-protein coupled receptor; Receptor; Transducer

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS7-0425-WR286.pdf

MSDS

MSDS - VS7-0425-WR286.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Isotype Control

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