Mouse Anti-LGR5 Recombinant Antibody (clone 349CT16.1.3.1)
CAT#: VS3-QX686
This product is a mouse antibody that recognizes LGR5. The antibody can be used in applications: WB, FC.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/1404_H2_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/103_B2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/102_B2_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Group enriched (Hep G2, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139292-LGR5
❮
❯
Specifications
- Immunogen
- A KLH conjugated synthetic peptide between 689-719 amino acids from human LGR5/GPR49.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- LGR5/GPR49
- Species Reactivity
- Human
- Clone
- 349CT16.1.3.1
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1:2000; FC: 1:25
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-199CT | Human Anti-LGR5 Recombinant Antibody (TAB-199CT) | ELISA, FC, RIA, IP, WB | Chimeric antibody (mouse/human) |
| TAB-199CT-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (TAB-199CT-S(P)) | ELISA, FC, RIA, IP, WB | Mouse scFv |
| TAB-199CT-F(E) | Human Anti-LGR5 Recombinant Antibody; Fab Fragment (TAB-199CT-F(E)) | ELISA, FC, RIA, IP, WB | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-196CT-S(P) | Rabbit Anti-LGR5 Recombinant Antibody; scFv Fragment (TAB-196CT-S(P)) | IHC, FC, ELISA, WB | Rabbit scFv |
| TAB-197CT-S(P) | Rabbit Anti-LGR5 Recombinant Antibody; scFv Fragment (TAB-197CT-S(P)) | IHC, FC, ELISA, WB | Rabbit scFv |
| TAB-198CT-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (TAB-198CT-S(P)) | ELISA, FC, RIA, IP, WB | Mouse scFv |
| TAB-200CT-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (TAB-200CT-S(P)) | ELISA, FC, RIA, IP, WB | Mouse scFv |
| TAB-201CT-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (TAB-201CT-S(P)) | ELISA, FC, RIA, IP, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2048 | Hi-Affi™ Recombinant Rabbit Anti-LGR5 Monoclonal Antibody (DS2048AB) | WB, IP, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0101-CN | Mouse Anti-LGR5 Recombinant Antibody (HPAB-0101-CN) | WB, ELISA, FC, IHC | Mouse IgG |
| HPAB-0105-CN | Human Anti-LGR5 Recombinant Antibody (HPAB-0105-CN) | WB, ELISA, FC, IHC | Chimeric (mouse/human) IgG1, κ |
| VS3-CJ177 | Rabbit Anti-LGR5 Recombinant Antibody (VS3-CJ177) | WB, IP, FC | Rabbit IgG |
| VS7-0425-WR285 | Mouse Anti-LGR5 Recombinant Antibody (clone 2B5B9) | FC | Mouse IgG2b |
| VS7-0425-WR286 | Mouse Anti-LGR5 Recombinant Antibody (clone 2B5C3) | WB, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0101-CN-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0101-CN-S(P)) | WB, ELISA, FC, IHC | Mouse scFv |
| HPAB-0102-CN-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0102-CN-S(P)) | WB, ELISA, FC, IHC | Mouse scFv |
| HPAB-0103-CN-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0103-CN-S(P)) | WB, ELISA, FC, IHC | Mouse scFv |
| HPAB-0104-CN-S(P) | Human Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0104-CN-S(P)) | WB, ELISA, FC, IHC | Human scFv |
| HPAB-0106-CN-S(P) | Human Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0106-CN-S(P)) | WB, ELISA, FC, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0101-CN-F(E) | Mouse Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0101-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| HPAB-0102-CN-F(E) | Mouse Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0102-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| HPAB-0103-CN-F(E) | Mouse Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0103-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| HPAB-0104-CN-F(E) | Human Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0104-CN-F(E)) | WB, ELISA, FC, IHC | Human Fab |
| HPAB-0106-CN-F(E) | Human Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0106-CN-F(E)) | WB, ELISA, FC, IHC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY32 | Mouse Anti-LGR5 Agonistic Antibody (VS-0724-XY32) | FC, Agonistic assays, CyTOF®, ICC | Mouse IgG2a |
| VS-0724-XY33 | Rat Anti-Lgr5 Agonistic Antibody (VS-0724-XY33) | FC, Agonistic assays, CyTOF®, ICC | Rat IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY173 | AbPlus™ Anti-LGR5 Magnetic Beads (VS-0424-XY173) | IP, Protein Purification | |
| VS-0724-YC625 | AbPlus™ Anti-LGR5 Magnetic Beads (VS-0724-YC625) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC71 | Rabbit Anti-LGR5 Antibody (VS-0924-YC71) - Cancer Stem Cell Marker | IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC634 | Recombinant Anti-LGR5 Vesicular Antibody, EV Displayed (VS-0425-YC634) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4011 | Anti-LGR5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4012 | Anti-Mouse LGR5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC118 | Recombinant Anti-LGR5 (AA 22-322 x AA 323-558) Biparatopic Antibody, Tandem scFv | ELISA, FC, IHC, WB | Tandem scFv |
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