Rabbit Anti-MAPK14 Recombinant Antibody (clone 6H8)
CAT#: ZG-0130U
Rabbit anti-MAPK14 antibody (clone 6H8) displayed high specificity against the MAPK14 protein of Human and Rat.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-MAPK14 Antibody (ZG-0130U) in WB
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, U-87 whole cell lysate, PC-3 whole cell lysate, L02 whole cell lysate, Rat Heart whole cell lysate. All lanes: p38 MAPK antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42, 42, 35, 36, 30 kDa
Observed band size: 42 kDa
IHC
Figure 2 Rabbit Anti-MAPK14 Antibody (ZG-0130U) in IHC
IHC image of ZG-0130U diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Rabbit Anti-MAPK14 Antibody (ZG-0130U) in IF
Immunofluorescence staining of Hela Cells with ZG-0130U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51825/834_A12_2_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_A_8_8.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_A_6_6.jpg
Normal Tissue
Figure 6 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_B_5_4.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112062-MAPK14
❮
❯
Specifications
- Immunogen
- A synthesized peptide derived from human p38 MAPK
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MAPK14
- Species Reactivity
- Human, Rat
- Clone
- 6H8
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Chlamydia and Alexander Disease
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA; mitogen-activated protein kinase 14; CSAID-binding protein; MAP kinase 14; MAP kinase Mxi2; MAP kinase p38 alpha; MAX-interacting protein 2; cytokine suppressive anti-inflammatory drug binding protein; mitogen-activated protein kinase p38 alpha; p38 MAP kinase; p38 mitogen activated protein kinase; p38alpha Exip; stress-activated protein kinase 2A
- Gene ID
- 1432
- UniProt ID
- Q16539
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0130U. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MAPK14"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0512MZ | Chicken Anti-RK Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2153 | Hi-Affi™ Recombinant Rabbit Anti-MAPK14 Monoclonal Antibody (DS2153AB) | ELISA, WB | IgG |
| MOR-4696 | Hi-Affi™ Recombinant Rabbit Anti-MAPK14 Monoclonal Antibody (TH210DS) | WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1145-CN | Recombinant Rabbit Anti-MAPK14 Monoclonal Antibody (CBACN-417) | WB, IF | Rabbit IgG |
| MRO-1146-CN | Recombinant Rabbit Anti-MAPK14 Monoclonal Antibody (JF55-07) | WB, IF, IHC | Rabbit IgG |
| ZG-0433U | Rabbit Anti-MAPK14 Recombinant Antibody (clone I15-E), FITC | FC | Rabbit IgG |
| VS3-FY886 | Recombinant Rabbit Anti-MAPK14 (phospho Thr180) Antibody (clone R08-4E6) | WB | Rabbit IgG |
| VS13-YC694 | Rabbit Anti-MAPK14 Recombinant Antibody (VS13-YC694) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1292 | AbPlus™ Anti-MAPK14 Magnetic Beads (VS-0724-YC1292) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4233 | Anti-MAPK14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4234 | Anti-Mouse MAPK14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4235 | Anti-Rat MAPK14 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: WB, IHC, ELISA, FC
Application: ELISA, FC, IF, IHC, WB
Application: ELISA, IHC, WB
-3.jpg)
Application: IB, FC, ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
