Rabbit Anti-MAPK3/MAPK1 Recombinant Antibody (VS3-FY2763) (CAT#: VS3-FY2763)
The anti-MAPK3 antibody is specifically engineered to target human ERK1/2 protein. It shows capability of cross-reacting with mouse and rat. This monoclonal antibody proves valuable in disease research related to B-Cell Lymphoma and Pleural Mesothelioma.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2683/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2683/if_selected.jpg
Figure 2 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_5_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_5_6.jpg
Figure 3 Breast
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/21384_B_3_4.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/21384_B_3_4.jpg
Figure 4 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_8.jpg
Figure 5 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_3.jpg
Figure 6 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_8_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_8_4.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_5.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000102882-MAPK3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000102882-MAPK3
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mitogen-activated protein kinase 3 of human, mouse and rat origins
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IP
- Conjugate
- Unconjugated, FITC
- Related Disease
- B-Cell Lymphoma and Pleural Mesothelioma
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Immunocytochemistry: 1/50-1/200
Target
- Alternative Names
- Mitogen-Activated Protein Kinase 3; Extracellular Signal-Regulated Kinase 1; Microtubule-Associated Protein 2 Kinase; Insulin-Stimulated MAP2 Kinase; MAP Kinase Isoform P44; EC 2.7.11.24; P44-ERK1; P44-MAPK; PRKM3; ERK-1; ERK1
- Gene ID
- 5595
- UniProt ID
- P27361
- Sequence Similarities
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
- Cellular Localization
- Cytoplasm
Nucleus
Membrane, caveola
Cell junction, focal adhesion
- Post Translation Modifications
- Phosphorylated upon KIT and FLT3 signaling (By similarity).
Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.
Ligand-activated ALK induces tyrosine phosphorylation.
Dephosphorylated by PTPRJ at Tyr-204.
Ubiquitinated by TRIM15 via 'Lys-63'-linked ubiquitination; leading to activation.
Deubiquitinated by CYLD.
Ubiquitination at Lys317
Modification sites at PhosphoSitePlus
- Protein Refseq
- NP_001035145.1; NP_001103361.1; NP_002737.2
- Function
- Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed:34497368).
MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade.
They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF.
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
FcεR1 Signaling Pathway
TCR Signaling Pathway
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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