Rabbit Anti-MAPK3/MAPK1 Recombinant Antibody (VS3-FY2763) (CAT#: VS3-FY2763)
The anti-MAPK3 antibody is specifically engineered to target human ERK1/2 protein. It shows capability of cross-reacting with mouse and rat. This monoclonal antibody proves valuable in disease research related to B-Cell Lymphoma and Pleural Mesothelioma.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2683/if_selected.jpg

(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_5_6.jpg

(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/21384_B_3_4.jpg

(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_8.jpg

(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_3.jpg

(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_8_4.jpg

(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_5.jpg

(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000102882-MAPK3
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mitogen-activated protein kinase 3 of human, mouse and rat origins
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IP
- Conjugate
- Unconjugated, FITC
- Related Disease
- B-Cell Lymphoma and Pleural Mesothelioma
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Immunocytochemistry: 1/50-1/200
Target
- Alternative Names
- Mitogen-Activated Protein Kinase 3; Extracellular Signal-Regulated Kinase 1; Microtubule-Associated Protein 2 Kinase; Insulin-Stimulated MAP2 Kinase; MAP Kinase Isoform P44; EC 2.7.11.24; P44-ERK1; P44-MAPK; PRKM3; ERK-1; ERK1
- Gene ID
- 5595
- UniProt ID
- P27361
- Sequence Similarities
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
- Cellular Localization
- Cytoplasm
Nucleus
Membrane, caveola
Cell junction, focal adhesion
- Post Translation Modifications
- Phosphorylated upon KIT and FLT3 signaling (By similarity).
Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.
Ligand-activated ALK induces tyrosine phosphorylation.
Dephosphorylated by PTPRJ at Tyr-204.
Ubiquitinated by TRIM15 via 'Lys-63'-linked ubiquitination; leading to activation.
Deubiquitinated by CYLD.
Ubiquitination at Lys317
Modification sites at PhosphoSitePlus
- Protein Refseq
- NP_001035145.1; NP_001103361.1; NP_002737.2
- Function
- Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed:34497368).
MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade.
They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF.
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "MAPK3"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0342MZ | Chicken Anti-MAPK3 Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
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For Research Use Only. Not For Clinical Use.
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