Rabbit Anti-MAPK3/MAPK1 Recombinant Antibody (VS3-FY2763)
CAT#: VS3-FY2763
The anti-MAPK3 antibody is specifically engineered to target human ERK1/2 protein. It shows capability of cross-reacting with mouse and rat. This monoclonal antibody proves valuable in disease research related to B-Cell Lymphoma and Pleural Mesothelioma.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2683/if_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_5_6.jpg
Normal Tissue
Figure 3 Breast
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/21384_B_3_4.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_8.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000102882-MAPK3
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mitogen-activated protein kinase 3 of human, mouse and rat origins
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IP
- Conjugate
- Unconjugated, FITC
- Related Disease
- B-Cell Lymphoma and Pleural Mesothelioma
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Immunocytochemistry: 1/50-1/200
Target
- Alternative Names
- Mitogen-Activated Protein Kinase 3; Extracellular Signal-Regulated Kinase 1; Microtubule-Associated Protein 2 Kinase; Insulin-Stimulated MAP2 Kinase; MAP Kinase Isoform P44; EC 2.7.11.24; P44-ERK1; P44-MAPK; PRKM3; ERK-1; ERK1
- Gene ID
- 5595
- UniProt ID
- P27361
- Sequence Similarities
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
- Cellular Localization
- Cytoplasm
Nucleus
Membrane, caveola
Cell junction, focal adhesion
- Post Translation Modifications
- Phosphorylated upon KIT and FLT3 signaling (By similarity).
Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.
Ligand-activated ALK induces tyrosine phosphorylation.
Dephosphorylated by PTPRJ at Tyr-204.
Ubiquitinated by TRIM15 via 'Lys-63'-linked ubiquitination; leading to activation.
Deubiquitinated by CYLD.
Ubiquitination at Lys317
Modification sites at PhosphoSitePlus
- Protein Refseq
- NP_001035145.1; NP_001103361.1; NP_002737.2
- Function
- Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed:34497368).
MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade.
They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF.
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Related Signaling Pathways
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MAPK3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0342MZ | Chicken Anti-MAPK3 Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2154 | Hi-Affi™ Recombinant Rabbit Anti-MAPK3 Monoclonal Antibody (DS2154AB) | WB, IP | IgG |
| MOR-4659 | Hi-Affi™ Recombinant Rabbit Anti-MAPK3 Monoclonal Antibody (TH173DS) | WB, IF, ICC, FC | IgG |
| MOR-4697 | Hi-Affi™ Recombinant Rabbit Anti-MAPK3 Monoclonal Antibody (TH211DS) | WB, IF, ICC, IHC-P, FC, ChIP, ELISA | IgG |
| MOR-0040-FY | Rabbit Anti-MAPK3 Recombinant Antibody (clone AFY0011) | IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0813F | Mouse Anti-MAPK3 Recombinant Antibody (clone E32) | WB, IP | Mouse IgG1 |
| VS7-0425-WR353 | Mouse Anti-MAPK3 Monoclonal Antibody (clone 1E5) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR354 | Mouse Anti-MAPK3 Monoclonal Antibody (VS7-0425-WR354) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
| VS7-0425-WR355 | Mouse Anti-MAPK3 Recombinant Antibody (clone 6C10) | WB, IHC-P, IF | Mouse IgG |
| VS7-0425-WR356 | Mouse Anti-MAPK3 Recombinant Antibody (clone 4G3) | IHC-P, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY181 | AbPlus™ Anti-MAPK3 Magnetic Beads (CBACN-216) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY190 | Mouse Anti-NHP MAPK3 Recombinant Antibody (clone 12D11) | WB, IHC, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4236 | Anti-MAPK3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4237 | Anti-Mouse MAPK3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4238 | Anti-Monkey MAPK3 Immunohistochemistry Kit | IHC |
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