Rabbit Anti-MCAM Antibody (clone 8A10) displayed high specificity against the MCAM protein of Human.
Figure 1 Rabbit Anti-MCAM Antibody (ZG-0463U) in WB
Western Blot
Positive WB detected in A375 whole cell lysate
All lanes CD146 antibody at 0.6μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 72 KDa
Observed band size: 120 KDa
Figure 2 Rabbit Anti-MCAM Antibody (ZG-0463U) in IHC
IHC image of ZG-0463U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Figure 3 Rabbit Anti-MCAM Antibody (ZG-0463U) in IF
Immunofluorescence staining of U251 cells with ZG-0463U at 1:37, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Figure 4 Rabbit Anti-MCAM Antibody (ZG-0463U) in FC
Overlay histogram showing A375 cells stained with ZG-0463U (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10, 000 events was performed.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1666z | Mouse Anti-MCAM Recombinant Antibody (clone 35D1) | WB, FC, IHC, IP | Mouse IgG1 |
MOB-0382CT | Recombinant Mouse anti-Human MCAM Monoclonal antibody (O2349) | IHC-P, WB | |
FAMAB-0018-CN | Mouse Anti-MCAM Recombinant Antibody (clone c264) | FC | Mouse IgG |
HPAB-0493LY | Human Anti-MCAM Recombinant Antibody (clone H5L0) | ELISA | Humanized IgG1 |
HPAB-0494LY | Human Anti-MCAM Recombinant Antibody (clone H10L2) | ELISA | Humanized IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-516CL | Anti-Human MCAM Recombinant Antibody (ABX-MA1) | WB, FuncS | Antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-441MZ | Mouse Anti-MCAM Recombinant Antibody (TAB-441MZ) | FC, FuncS | Mouse IgG1 |
TAB-441MZ-S(P) | Mouse Anti-MCAM Recombinant Antibody; scFv Fragment (TAB-441MZ-S(P)) | FC, WB, IHC | Mouse scFv |
TAB-441MZ-F(E) | Mouse Anti-MCAM Recombinant Antibody; Fab Fragment (TAB-441MZ-F(E)) | FC, WB, IHC | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-442MZ | Human Anti-MCAM Recombinant Antibody (TAB-442MZ) | FC, WB, IHC | Chimeric (Mouse/Human) IgG1 |
TAB-442MZ-S(P) | Human Anti-MCAM Recombinant Antibody; scFv Fragment (TAB-442MZ-S(P)) | FC, WB, IHC | Human scFv |
TAB-443MZ-S(P) | Human Anti-MCAM Recombinant Antibody; scFv Fragment (TAB-443MZ-S(P)) | FC, WB, IHC | Human scFv |
TAB-442MZ-F(E) | Human Anti-MCAM Recombinant Antibody; Fab Fragment (TAB-442MZ-F(E)) | FC, WB, IHC | Chimeric (Mouse/Human) Fab |
TAB-443MZ-F(E) | Human Anti-MCAM Recombinant Antibody; Fab Fragment (TAB-443MZ-F(E)) | FC, WB, IHC | Chimeric (Mouse/Human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-444MZ | Anti-Human MCAM Recombinant Antibody (huAA98) | ELISA | Humanized antibody |
TAB-444MZ-S(P) | Anti-Human MCAM Recombinant Antibody scFv Fragment (huAA98) | ELISA | Humanized antibody |
TAB-444MZ-F(E) | Anti-Human MCAM Recombinant Antibody Fab Fragment (huAA98) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2188 | Hi-Affi™ Recombinant Rabbit Anti-MCAM Monoclonal Antibody (DS2188AB) | ICC/IF, IHC-Fr, WB, IP, IHC-P, ICC, FC | IgG |
MOR-4644 | Hi-Affi™ Recombinant Rabbit Anti-MCAM Monoclonal Antibody (TH157DS) | WB | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0018-CN-S(P) | Mouse Anti-MCAM Recombinant Antibody (clone c264); scFv Fragment | FC | Mouse scFv |
HPAB-0031-YJ-S(P) | Mouse Anti-MCAM Recombinant Antibody (clone AA98); scFv Fragment | ELISA, FC | Mouse scFv |
HPAB-1127WJ-S(P) | Mouse Anti-MCAM Recombinant Antibody (clone C3.19.1); scFv Fragment | ELISA | Mouse scFv |
HPAB-1128WJ-S(P) | Mouse Anti-MCAM Recombinant Antibody (clone C6.11.3); scFv Fragment | ELISA | Mouse scFv |
HPAB-1129WJ-S(P) | Mouse Anti-MCAM Recombinant Antibody (clone C3.10); scFv Fragment | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0018-CN-F(E) | Mouse Anti-MCAM Recombinant Antibody (clone c264); Fab Fragment | FC | Mouse Fab |
HPAB-0493LY-F(E) | Human Anti-MCAM Recombinant Antibody (clone H5L0); Fab Fragment | ELISA | Humanized Fab |
HPAB-0494LY-F(E) | Human Anti-MCAM Recombinant Antibody (clone H10L2); Fab Fragment | ELISA | Humanized Fab |
HPAB-0495LY-F(E) | Human Anti-MCAM Recombinant Antibody (clone H14L0); Fab Fragment | ELISA | Humanized Fab |
HPAB-0496LY-F(E) | Human Anti-MCAM Recombinant Antibody (clone H11L3); Fab Fragment | ELISA | Humanized Fab |
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