Mouse Anti-MCL1 Recombinant Antibody (ZG-0272C)
CAT#: ZG-0272C
This product is a mouse antibody that recognizes MCL1. It can be used in a wide variety of applications, such as: WB, IHC, IF, ELISA.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-MCL1 Antibody (ZG-0272C) in WB
Western Blot analysis using Mcl-1 monoclonal antibody against HeLa (1), BCBL-1 (2), Jurkat (3) and HL60 (4) cell lysate.
IHC
Figure 2 Mouse Anti-MCL1 Antibody (ZG-0272C) in IHC
Immunohistochemistry analysis of paraffin-embedded human lymphnode tissues with DAB staining using Mcl-1 monoclonal antibody.
IF
Figure 3 Mouse Anti-MCL1 Antibody (ZG-0272C) in IF
Confocal immunofluorescence analysis of HepG2 cells using MCL1 monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8455/if_selected.jpg
Normal Tissue
Figure 2 Appendix
High expression in appendix
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8455/22739_A_1_2_val_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31125/65497_A_3_1_selected.jpg
Normal Tissue
Figure 4 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68195/ihc_selected.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31125/65497_A_7_3.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31125/65497_A_6_6.jpg
Normal Tissue
Figure 7 Thyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31125/65497_B_3_5.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (U-698)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143384-MCL1
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Specifications
- Immunogen
- Purified recombinant fragment of human MCL-1 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MCL1
- Species Reactivity
- Human
- Applications
- WB, IHC, IF, ELISA
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- WB 1:500-1:2000. IHC 1:200-1:1000. IF 1:200-1:1000. ELISA 1:10000.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MCL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1225CT | Recombinant Mouse anti-Human MCL1 Monoclonal antibody (49H4) | IHC-P | |
| MRO-0971-CN | Recombinant Rabbit Anti-MCL1 Monoclonal Antibody (CBACN-355) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY906 | Recombinant Rabbit Anti-MCL1 Antibody (clone R04-3A4) | WB, ICC, IF, IP | Rabbit IgG |
| VS7-0425-WR366 | Mouse Anti-MCL1 Recombinant Antibody (VS7-0425-WR366) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
| VS7-0425-WR367 | Mouse Anti-MCL1 Recombinant Antibody (clone 8C6D4B1) | WB, IHC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2189 | Hi-Affi™ Recombinant Rabbit Anti-MCL1 Monoclonal Antibody (DS2189AB) | ICC, IF, WB, IHC-P, ICC, FC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1025 | PE-A*03:01/Human MCL1 (RLLFFAPTR) MHC Tetramer | FCM, IHC | |
| MHC-CN1026 | APC-A*03:01/Human MCL1 (RLLFFAPTR) MHC Tetramer | FCM, IHC | |
| MHC-CN1027 | FITC-A*03:01/Human MCL1 (RLLFFAPTR) MHC Tetramer | FCM, IHC | |
| MHC-CN1028 | A*03:01/Human MCL1 (RLLFFAPTR) MHC Tetramer | FCM, IHC | |
| MHC-YF546 | A*01:01/Human MCL-1 (QSLEIISRY) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBS-0143 | Human Anti-MCL1 Recombinant Antibody (Clone 01JF43); Fab Fragment | ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBS-0143 | Human Anti-MCL1 Recombinant Antibody (Clone 01JF43); scFv Fragment | ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1318 | Anti-MCL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4301 | Anti-Mouse MCL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4300 | Anti-Human MCL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4302 | Anti-Rat MCL1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC710 | CytoStream™ Rabbit Anti-MCL1 Recombinant Antibody (VS13-YC710) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC402 | Recombinant Anti-MCL1 Vesicular Antibody, EV Displayed (VS-0425-YC402) | ELISA, FC, Cell-uptake |
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