Mouse Anti-MUC5AC Recombinant Antibody (clone 8H8G6)
CAT#: VS7-0425-WR441
This product is a mouse IgG2a antibody that binds to human MUC5AC. The antibody 8H8G6 recognizes specific amino acid domain of human MUC5AC (AA: 5528-5627). It can be used for IHC and FC assays.
Gene Expression
Normal Tissue
Figure 1 High expression in stomach 1
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40615/124256_A_6_1_val_selected.jpg
Normal Tissue
Figure 2 Low expression in liver
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40615/124256_A_9_4_val_selected.jpg
Normal Tissue
Figure 3 Stomach
Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40615/124256_A_3_1.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (A549, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000215182-MUC5AC
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Specifications
- Immunogen
- Recombinant human MUC5AC (AA: 5528-5627) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human MUC5AC
- Species Reactivity
- Human
- Clone
- 8H8G6
- Molecule Class
- Mucin
- Applications
- IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MUC5AC; Mucin 5AC, Oligomeric Mucus/Gel-Forming Pseudogene; Lewis B Blood Group Antigen; Mucin-5AC; Gastric Mucin; Tracheobronchial Mucin; Mucin; Mucin 5, Subtypes A And C, Tracheobronchial/Gastric; Mucin 5AC, Oligomeric Mucus/Gel-Forming; MUC5; TBM; Majo
- Gene ID
- 4586
- UniProt ID
- P98088
- Long Name
- Mucin 5AC, oligomeric mucus/gel-forming
- Cellular Localization
- Secreted
- Post Translation Modifications
- Disulfide bond; Glycoprotein
- Protein Refseq
- NP_001291288.1
- Function
- Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed:14535999, PubMed:14718370).; Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed:14535999).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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