Mouse Anti-NCAM1 Recombinant Antibody (clone 1F6F10)
CAT#: VS7-0425-WR457
This product is a mouse IgG1 antibody that binds to human NCAM1. The antibody 1F6F10 recognizes specific amino acid domain of human NCAM1 (AA: 568-708). It can be used for WB assay. It cross reacts with mouse.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39835/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuropil
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39835/100448_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Peripheral nerve/ganglion
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39835/100448_A_8_3.jpg
Normal Tissue
Figure 4 Soft tissue
Peripheral nerve
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39835/100448_B_1_7.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HEL, RH-30, SCLC-21H, SH-SY5Y, U-138 MG, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000149294-NCAM1
❮
❯
Specifications
- Immunogen
- Recombinant human NCAM1 (AA: 568-708) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NCAM1
- Species Reactivity
- Human, Mouse
- Clone
- 1F6F10
- Molecule Class
- Cell Adhesion Molecule
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- CD56; NCAM; MSK39; neural cell adhesion molecule 1; antigen recognized by monoclonal antibody 5.1H11; neural cell adhesion molecule, NCAM
- Gene ID
- 4684
- UniProt ID
- P13591
- Long Name
- Neural Cell Adhesion Molecule 1
- Cellular Localization
- Cell membrane; Membrane; Secreted
- Post Translation Modifications
- Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein; Phosphoprotein
- Protein Refseq
- NP_000606.3
- Function
- Host cell receptor for virus entry; Receptor
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
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Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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