Mouse Anti-NFKB2 Recombinant Antibody (clone 2F10A7) (CAT#: VS7-0425-WR478)
This product is a mouse IgG2b antibody that binds to human NFKB2. The antibody 2F10A7 recognizes specific amino acid domain of human NFKB2 (AA: 712-900). It can be used for FC assay. It cross reacts with mouse.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/if_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22098/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22098/if_selected.jpg
Figure 2 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong positivity in enocrine and exocrine cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22098/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows strong positivity in enocrine and exocrine cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22098/ihc_selected.jpg
Figure 4 Appendix
(High expression in appendix)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_1_2_val_selected.jpg
(High expression in appendix)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_1_2_val_selected.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_7_5.jpg
Figure 6 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_9_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_9_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000077150-NFKB2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000077150-NFKB2
Specifications
- Immunogen
- Recombinant human NFKB2 (AA: 712-900) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human NFKB2
- Species Reactivity
- Human
- Clone
- 2F10A7
- Molecule Class
- NF-κB Transcription Factor
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Immunodeficiency, common variable, 10 (CVID10)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NFKB2; LYT-10; NFKB, P52/P100 Subunit; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells 2; Nuclear Factor NF-Kappa-B P52 Subunit; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2; CVID10; Transcription Factor NFKB2; Nuclear
- Gene ID
- 4791
- UniProt ID
- Q00653
- Long Name
- Nuclear Factor Kappa B Subunit 2
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001070962.1
- Function
- Activator; DNA-binding; Repressor
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
BCR Signaling Pathway
cAMP Signaling Pathway
NLR Signaling Pathway
TCR Signaling Pathway
TLR Signaling Pathway
TNF Signaling Pathway
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1185CT | Recombinant Mouse anti-Human NFKB2 Monoclonal antibody (0.U.284) | GSA, IP, WB | |
| MRO-1089-CN | Recombinant Rabbit Anti-NFKB2 Monoclonal Antibody (JM82-03) | WB, IF, IHC, IP | Rabbit IgG |
| FAMAB-1228CQ | Mouse Anti-NFKB2 Recombinant Antibody (clone SAIC-26C-15) | Immuno-MRM, ELISA | Mouse IgG1 |
| VS3-FY1034 | Recombinant Rabbit Anti-NFKB2 Antibody (clone R01-8B2) | WB, ICC, IF, IP | Rabbit IgG |
| VS7-0425-WR477 | Mouse Anti-NFKB2 Recombinant Antibody (clone 5E6A12) | WB, ELISA | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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