Recombinant Rabbit Anti-NFKB2 Antibody (clone R01-8B2)
CAT#: VS3-FY1034
This product is a recombinant rabbit antibody that recognizes NFKB2. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R01-8B2 is specific for human, mouse NFKB2. The antigen is synthetic peptide of human nfkb p100/nfkb2.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong positivity in enocrine and exocrine cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22098/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22098/if_selected.jpg
Normal Tissue
Figure 4 Appendix
High expression in appendix
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_1_2_val_selected.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_7_5.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000077150-NFKB2
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WB
Figure 1. Recombinant Rabbit Anti-NFKB2 Antibody (clone R01-8B2) in WB.
Western Blot analysis of NF-KB p100/NF-KB2 in K562, Hela lysates using NF-KB p100 Antibody.
ICC
Figure 2. Recombinant Rabbit Anti-NFKB2 Antibody (clone R01-8B2) in ICC.
Immunocytochemical analysis of NF-KB p100/NF-KB2 (green) in HeLa using NF-KB p100/NF-KB2 Antibody and DAPI (blue).
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Specifications
- Immunogen
- Synthetic peptide of human NFkB p100/NFKB2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse NFKB2
- Species Reactivity
- Human, Mouse
- Clone
- R01-8B2
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 97 kDa; Observed MW: 120 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Nuclear Factor Kappa B Subunit 2; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2 (P49/P100); Lymphocyte Translocation Chromosome 10 Protein; DNA-Binding Factor KBF2; Oncogene Lyt-10; H2TF1; LYT10; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells 2; Nuclear Factor NF-Kappa-B P100 Subunit; Nuclear Factor NF-Kappa-B P52 Subunit
- Gene ID
- 4791
- UniProt ID
- Q00653
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Related Signaling Pathways
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Related Diseases
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NFKB2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4053z | Mouse Anti-NFKB2 Recombinant Antibody (clone 29B2) | WB, IP, IF, FC | Mouse IgG |
| MOB-1185CT | Recombinant Mouse anti-Human NFKB2 Monoclonal antibody (0.U.284) | GSA, IP, WB | |
| MRO-1089-CN | Recombinant Rabbit Anti-NFKB2 Monoclonal Antibody (JM82-03) | WB, IF, IHC, IP | Rabbit IgG |
| FAMAB-1228CQ | Mouse Anti-NFKB2 Recombinant Antibody (clone SAIC-26C-15) | Immuno-MRM, ELISA | Mouse IgG1 |
| VS13-YC798 | Rabbit Anti-NFKB2 Recombinant Antibody (VS13-YC798) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2434 | Hi-Affi™ Recombinant Rabbit Anti-NFKB2 Monoclonal Antibody (DS2434AB) | IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1495 | Anti-NFKB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4819 | Anti-Mouse NFKB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4818 | Anti-Human NFKB2 Immunohistochemistry Kit | IHC |
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