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    Mouse Anti-NHP TLR4 Recombinant Antibody (clone HTA125) (CAT#: VS-1024-XY505)

    Specifically targeting TLR4, this mouse monoclonal antibody showcases reactivity with dog, cat, guinea pig, human, mouse, non-human primate, pig, and rhesus monkey samples. The immunogen is derived from the Ba/F3 cell line expressing TLR4, ensuring precise antigen recognition. Optimized for flow cytometry (FC) applications, this antibody is an essential tool for investigating TLR4's role in immune response and signal transduction across a variety of species, enhancing our understanding of innate immunity in diverse biological contexts.

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    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & the Golgi apparatus.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/912_G5_2_selected.jpg
    Figure 2 IHC staining of human spleen
    Figure 2 IHC staining of human spleen
    (Immunohistochemical staining of human spleen shows distinct cytoplasmic positivity in subsets of cells in the red pulp.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/ihc_selected.jpg
    Figure 3 IF staining of human cell line U-251 MG
    Figure 3 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/911_G5_4_blue_red_green.jpg
    Normal Tissue
    Figure 4 Lymph node
    Figure 4 Lymph node
    (Non-germinal center cells
    Staining:

    Medium

    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/
    membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/140817_A_8_8.jpg
    Figure 5 Spleen
    Figure 5 Spleen
    (Cells in red pulp
    Staining:

    Medium

    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/
    membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/140817_B_7_4.jpg
    RNA Expression
    Figure 6 RNA cell line category: Cell line enhanced (Karpas-707, TIME)
    Figure 6 RNA cell line category: Cell line enhanced (Karpas-707, TIME)
    (Cell lines ordered by descending RNA expression order.)
    * https://v21.proteinatlas.org/ENSG00000136869-TLR4
    Figure 6 RNA cell line category: Cell line enhanced (Karpas-707, TIME)
    Figure 6 RNA cell line category: Cell line enhanced (Karpas-707, TIME)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136869-TLR4

    Specifications

    • Immunogen
    • Ba/F3 cell line expressing TLR4
    • Host Species
    • Mouse
    • Type
    • Mouse IgG2a
    • Specificity
    • TLR4
    • Species Reactivity
    • Dog, Cat, Guinea pig, Human, Mouse, Non-human primate, Pig, Rhesus monkey
    • Clone
    • HTA125
    • Applications
    • FC

    Product Property

    • Purification
    • Protein A purified
    • Purity
    • >95%, determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • PBS, pH 7.4
    • Storage
    • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

    Applications

    • Application Notes
    • FC: 1:10-1:25

    Target

    • Alternative Names
    • TOLL; CD284; TLR-4; ARMD10

    Recommended Products

    Recommended Dilution Buffer

    Isotype Control

    Secondary Antibody

    Related Resources

    • Related Signaling Pathways
    • Related Diseases

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    Humanized Antibody Products Fab Antibody Products IgG Antibody Products Human Antibody Products Chimeric Antibody Products Blocking Antibody Products Neutralizing Antibody Products ScFv Antibody Products Antibody Magnetic Beads Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research ScFv-Fc Chimera Products Extracellular Vesicle (EV) Antibody Products
    CAT Product Name Application Type
    TAB-613CL Human Anti-TLR4 Recombinant Antibody (TAB-613CL) FuncS Human IgG

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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