Mouse Anti-NHP TLR4 Recombinant Antibody (clone HTA125)
CAT#: VS-1024-XY505
Specifically targeting TLR4, this mouse monoclonal antibody showcases reactivity with dog, cat, guinea pig, human, mouse, non-human primate, pig, and rhesus monkey samples. The immunogen is derived from the Ba/F3 cell line expressing TLR4, ensuring precise antigen recognition. Optimized for flow cytometry (FC) applications, this antibody is an essential tool for investigating TLR4's role in immune response and signal transduction across a variety of species, enhancing our understanding of innate immunity in diverse biological contexts.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/912_G5_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human spleen
Immunohistochemical staining of human spleen shows distinct cytoplasmic positivity in subsets of cells in the red pulp.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/911_G5_4_blue_red_green.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/140817_A_8_8.jpg
Normal Tissue
Figure 5 Spleen
Cells in red pulp
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/140817_B_7_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Karpas-707, TIME)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Karpas-707, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136869-TLR4
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Specifications
- Immunogen
- Ba/F3 cell line expressing TLR4
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- TLR4
- Species Reactivity
- Dog, Cat, Guinea pig, Human, Mouse, Non-human primate, Pig, Rhesus monkey
- Clone
- HTA125
- Applications
- FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- FC: 1:10-1:25
Target
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Related Signaling Pathways
NLR Signaling Pathway
TLR Signaling Pathway
Related Diseases
Rheumatoid Arthritis
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone HTA125"
- CAT
- Product Name
See other products for "TLR4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| PABL-729 | Human Anti-TLR4 Recombinant Antibody (PABL-729) | Neut | Human IgG |
| MOB-2059CT | Recombinant Mouse anti-Human TLR4 Monoclonal antibody (1203B) | ELISA, WB | |
| FN-223CQ | Mouse Anti-TLR4 Recombinant Antibody (VMC213) | FC, FuncS | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-613CL | Human Anti-TLR4 Recombinant Antibody (TAB-613CL) | FuncS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-722 | Human Anti-TLR4 Recombinant Antibody; Fab Fragment (PFBL-722) | Neut | Human Fab |
| HPAB-0318CQ-F(E) | Rat Anti-Tlr4 Recombinant Antibody Fab Fragment (HPAB-0318CQ-F(E)) | FC, IP, Apop | Rat Fab |
| HPAB-AP199-YC-F(E) | Human Anti-TLR4 Recombinant Antibody; Fab Fragment (HPAB-AP199-YC-F(E)) | ELISA, FC, Inhib | Human Fab |
| HPAB-AP200-YC-F(E) | Human Anti-TLR4 Recombinant Antibody; Fab Fragment (HPAB-AP200-YC-F(E)) | ELISA, FC, Inhib | Human Fab |
| HPAB-0678YY-F(E) | Human Anti-TLR4 Recombinant Antibody (clone CBT111YJ); Fab Fragment | ELISA, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-722 | Human Anti-TLR4 Recombinant Antibody; scFv Fragment (PSBL-722) | Neut | Human scFv |
| HPAB-AP199-YC-S(P) | Human Anti-TLR4 Recombinant Antibody; scFv Fragment (HPAB-AP199-YC-S(P)) | ELISA, FC, Inhib | Human scFv |
| HPAB-AP200-YC-S(P) | Human Anti-TLR4 Recombinant Antibody; scFv Fragment (HPAB-AP200-YC-S(P)) | ELISA, FC, Inhib | Human scFv |
| HPAB-AP201-YC-S(P) | Human Anti-TLR4 Recombinant Antibody; scFv Fragment (HPAB-AP201-YC-S(P)) | ELISA, FC, Inhib | Human scFv |
| HPAB-AP202-YC-S(P) | Human Anti-TLR4 Recombinant Antibody; scFv Fragment (HPAB-AP202-YC-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-181 | Human Anti-TLR4 Recombinant Antibody (PABX-181) | WB, ELISA, FuncS | Human IgG |
| PABX-181-F (E) | Human Anti-TLR4 Recombinant Antibody; Fab Fragment (PABX-181-F (E)) | WB, ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-181-S (P) | Human Anti-TLR4 Recombinant Antibody; scFv Fragment (PABX-181-S (P)) | WB, ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2036CQ | Recombinant Rat Anti-Tlr4 Antibody (MTS510) | FC, IHC, ICFC, IF, IP, BL | IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2037CQ | Mouse Anti-TLR4 Recombinant Antibody (clone 3C3) | Neut, FC | Mouse IgG1 |
| NEUT-2038CQ | Mouse Anti-TLR4 Recombinant Antibody (clone CBL961) | FC, ICC, IF, IP, Neut | Mouse IgG2a, κ |
| NEUT-2039CQ | Mouse Anti-TLR4 Recombinant Antibody (clone HTA125) | ICC, IP, FC, Neut | Mouse IgG2a |
| NEUT-2040CQ | Mouse Anti-TLR4 Recombinant Antibody (NEUT-2040CQ) | Neut | Mouse IgG1 |
| NEUT-2041CQ | Mouse Anti-TLR4 Recombinant Antibody (clone 7E3) | Neut, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY255 | AbPlus™ Anti-TLR4 Magnetic Beads (HTA125) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1162 | CytoStream™ Rabbit Anti-TLR4 Polyclonal Antibody (VS13-YC1162) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY30 | Human Anti-TLR4 scFv-Fc Chimera (VS-0425-FY30) | FC, ELISA, Neut | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC502 | Recombinant Anti-TLR4 Vesicular Antibody, EV Displayed (VS-0425-YC502) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7306 | Anti-TLR4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7307 | Anti-Mouse TLR4 Immunohistochemistry Kit | IHC |
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