Human Anti-TLR4 Recombinant Antibody (TAB-613CL)

Name: Human Anti-TLR4 Recombinant Antibody (TAB-613CL)
SKU: TAB-613CL
Rating: 5 (1 reviews)

CAT#: TAB-613CL

This product is a recombinant human antibody that recognizes TLR4. The antibody was purified by affinity chromatography.

Tested Data Gene Expression

SDS-PAGE

Figure 1 Anti-Human TLR4 Recombinant Antibody (TAB-613CL) in SDS-PAGE

SDS-PAGE analysis of TAB-613CL in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.

SEC-HPLC

Figure 2 Anti-Human TLR4 Recombinant Antibody (TAB-613CL) in HPLC

The purity of TAB-613CL was greater than 95% as determined by SEC-HPLC.

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/912_G5_2_selected.jpg

Subcellular Location Figure 2 IHC staining of human spleen

Immunohistochemical staining of human spleen shows distinct cytoplasmic positivity in subsets of cells in the red pulp.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/ihc_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/911_G5_4_blue_red_green.jpg

Normal Tissue Figure 4 Lymph node

Non-germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/140817_A_8_8.jpg

Normal Tissue Figure 5 Spleen

Cells in red pulp
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/140817_B_7_4.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (Karpas-707, TIME)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (Karpas-707, TIME)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136869-TLR4

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Specifications

Host Species
Human

Derivation
Humanized

Type
Human IgG

Specificity
Human

Species Reactivity
Human

Applications
FuncS

Related Disease
Inflammatory Diseases

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
TLR4; toll-like receptor 4; TOLL; CD284; TLR-4; ARMD10; hToll; homolog of Drosophila toll

Gene ID
7099

UniProt ID
O00206

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Related Signaling Pathways

NLR Signaling Pathway

TLR Signaling Pathway

Related Diseases

Rheumatoid Arthritis

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-613CL.pdf

MSDS

MSDS - TAB-613CL.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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