Human Anti-TLR4 scFv-Fc Chimera (VS-0425-FY30)
CAT#: VS-0425-FY30
The anti-TLR4 scFv-Fc antibody is a monoclonal antibody that specifically binds to Toll-like Receptor 4 (TLR4), a pattern recognition receptor involved in innate immune responses. TLR4 is known to play a role in the pathogenesis of various diseases, including inflammatory conditions and certain types of cancer. The antibody's specificity for TLR4 makes it a valuable tool for both research and therapeutic applications. The anti-TLR4 scFv-Fc antibody represents a promising therapeutic and research tool with the potential to address a wide range of diseases driven by TLR4 activation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/912_G5_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human spleen
Immunohistochemical staining of human spleen shows distinct cytoplasmic positivity in subsets of cells in the red pulp.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/911_G5_4_blue_red_green.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/140817_A_8_8.jpg
Normal Tissue
Figure 5 Spleen
Cells in red pulp
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/140817_B_7_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Karpas-707, TIME)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Karpas-707, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136869-TLR4
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Specifications
- Immunogen
- Human TLR4
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human TLR4
- Species Reactivity
- Human
- Applications
- Flow Cytometry, ELISA, Neutralization
- Related Disease
- Macular Degeneration, Age-Related, 10 and Pertussis
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- Toll Like Receptor 4; Toll-Like Receptor 4; HToll; Homolog Of Drosophila Toll; CD284 Antigen; ARMD10; CD284; TLR-4; TOLL
- Gene ID
- 7099
- UniProt ID
- O00206
- Long Name
- Toll Like Receptor 4
- Sequence Similarities
- Belongs to the Toll-like receptor family.
- Cellular Localization
- Cell membrane; Single-pass type I membrane protein; Early endosome; Cell projection, ruffle
- Post Translation Modifications
- N-glycosylated.
Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response.
Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
Phosphorylated on tyrosine residues by LYN after binding lipopolysaccharide.
Glycosylation at Asn205, Asn35, Asn282, Asn309, Asn173, Asn497, Asn526, Asn575, Asn624, and Asn630
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 11 sites, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_003257.1; NP_612564.1; NP_612567.1
- Function
- Transmembrane receptor that functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways.
At the plasma membrane, cooperates with LY96 to mediate the innate immune response to bacterial lipopolysaccharide (LPS).
Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+).
Mechanistically, acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Alternatively, CD14-mediated TLR4 internalization via endocytosis is associated with the initiation of a MYD88-independent signaling via the TICAM1-TBK1-IRF3 axis leading to type I interferon production.
In addition to the secretion of proinflammatory cytokines, initiates the activation of NLRP3 inflammasome and formation of a positive feedback loop between autophagy and NF-kappa-B signaling cascade.
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Related Signaling Pathways
NLR Signaling Pathway
TLR Signaling Pathway
Related Diseases
Rheumatoid Arthritis
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| HPAB-AP201-YC-F(E) | Human Anti-TLR4 Recombinant Antibody; Fab Fragment (HPAB-AP201-YC-F(E)) | ELISA, FC, Inhib | Human Fab |
| HPAB-AP202-YC-F(E) | Human Anti-TLR4 Recombinant Antibody; Fab Fragment (HPAB-AP202-YC-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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