Rabbit Anti-NT5E Recombinant Antibody (clone 6F6) (CAT#: ZG-0490U)
Rabbit Anti-NT5E Antibody (clone 6F6) displayed high specificity against the NT5E protein of Human.
Specific Inquiry
IHC
Figure 1 Rabbit Anti-NT5E Antibody (ZG-0490U) in IHC
IHC image of ZG-0490U diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 2 Rabbit Anti-NT5E Antibody (ZG-0490U) in IHC
IHC image of ZG-0490U diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human CD73
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NT5E
- Species Reactivity
- Human
- Clone
- 6F6
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
- Related Disease
- Calcification Of Joints And Arteries and Intestinal Tuberculosis.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry. Specific conditions should be optimized by the end user.
IHC:1:50-1:200
Target
- Alternative Names
- 5'-nucleotidase; 5'-NT; EC 3.1.3.5; Ecto-5'-nucleotidase; CD antigen CD73; NT5E; NT5 NTE
- Gene ID
- 4907
- UniProt ID
- P21589
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1936z | Mouse Anti-NT5E Recombinant Antibody (MOB-1936z) | WB, IHC, IF | Mouse IgG1 |
| MRO-0315-CN | Recombinant Rabbit Anti-NT5E Monoclonal Antibody (JM11-40) | WB, IHC | Rabbit IgG |
| MRO-1759-CN | Rabbit Anti-NT5E Polyclonal Antibody (MRO-1759-CN) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-M0172-YC | Mouse Anti-NT5E Recombinant Antibody (HPAB-M0172-YC) | FuncS | Mouse IgG |
| FAMAB-1684CQ | Rabbit Anti-Nt5e Recombinant Antibody (clone YDBYT 208.3) | ELISA | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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