Rabbit Anti-PDGFRB (pY740) Recombinant Antibody (clone R06-8X3)
CAT#: VS3-WK2138
This product is a rabbit recombinant antibody that is specific to phosphorylated PDGFRB. The target of this antibody is phospho-PDGFRB (Tyr740). The clone R06-8X3 can be used in WB analysis at a dilution of 1/500-1/1000 and IF analysis at a dilution of 1/50-1/200. The molecular weight of PDGFRB (pTyr740) observed in WB experiments using this antibody is 190 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line ASC TERT1
Immunofluorescent staining of human cell line ASC TERT1 shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28499/1774_C4_13_cr5950fa3646972_selected.jpg
Normal Tissue
Figure 2 Testis
Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_A_4_6.jpg
Normal Tissue
Figure 3 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_A_2_7.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_B_9_5.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (ASC diff, ASC TERT1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113721-PDGFRB
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Tyr740 of human, mouse and rat PDGFRB
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-8X3
- Applications
- WB, ICC, IF
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
Target
- Alternative Names
- Platelet Derived Growth Factor Receptor Beta; Platelet-Derived Growth Factor Receptor, Beta Polypeptide; Beta-Type Platelet-Derived Growth Factor Receptor; Platelet-Derived Growth Factor Receptor 1; CD140 Antigen-Like Family Member B; PDGF-R-Beta; EC 2.7.10.1; PDGFR-Beta; PDGFR-1; PDGFR1; PDGFR; Platelet-Derived Growth Factor Receptor Beta
- Gene ID
- 5159
- UniProt ID
- P09619
- Long Name
- Platelet Derived Growth Factor Receptor Beta
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
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