Mouse Anti-PSCA Recombinant Antibody (clone 5H5A7) (CAT#: VS7-0425-WR699)
This product is a mouse IgG2a antibody that binds to human PSCA. The antibody 5H5A7 recognizes specific amino acid domain of human PSCA (AA: 1-114). It can be used for ICC and FC assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30783/1550_B1_8_selected.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30783/1550_B1_8_selected.jpg
Figure 2 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in subset of renal tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in subset of renal tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/ihc_selected.jpg
Figure 3 Stomach
(Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/132825_A_6_1.jpg
(Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/132825_A_6_1.jpg
Figure 4 Colon
(Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/132825_A_9_3.jpg
(Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/132825_A_9_3.jpg
Figure 5 Kidney
(Cells in tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/132825_A_7_5.jpg
(Cells in tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/132825_A_7_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (CAPAN-2, HeLa, OE19, RT4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167653-PSCA/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167653-PSCA/subcellular
Specifications
- Immunogen
- Recombinant human PSCA (AA: 1-114) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human PSCA
- Species Reactivity
- Human
- Clone
- 5H5A7
- Molecule Class
- Glycosylphosphatidylinositol (Gpi)-Anchored Cell Surface Protein
- Applications
- ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PSCA; PRO232; Prostate Stem Cell Antigen
- Gene ID
- 8000
- UniProt ID
- O43653
- Long Name
- Prostate stem cell antigen
- Cellular Localization
- Cell membrane; Membrane
- Post Translation Modifications
- Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein
- Protein Refseq
- NP_005663.2
- Function
- May be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro.; May act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro inhibits nicotine-induced signaling probably implicating alpha-3:beta-2- or alpha-7-containing nAChRs.
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1981z | Mouse Anti-PSCA Recombinant Antibody (clone 24F5) | WB, FC, IF | Mouse IgG1, κ |
| HPAB-0286CQ | Human Anti-PSCA Recombinant Antibody (clone mAb 5F2) | FC, IHC, FuncS | Human IgG1 |
| HPAB-1309WJ | Mouse Anti-PSCA Recombinant Antibody (clone 6B8) | ELISA, WB | Mouse IgG |
| HPAB-1900-FY | Human Anti-PSCA Recombinant Antibody (HPAB-1900-FY) | FC, Inhib, WB, ELISA | Human IgG1 |
| VS7-0425-WR700 | Mouse Anti-PSCA Recombinant Antibody (clone 3F4E4) | FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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