Mouse Anti-PSCA Recombinant Antibody (clone 5H5A7)
CAT#: VS7-0425-WR699
This product is a mouse IgG2a antibody that binds to human PSCA. The antibody 5H5A7 recognizes specific amino acid domain of human PSCA (AA: 1-114). It can be used for ICC and FC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30783/1550_B1_8_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in subset of renal tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/ihc_selected.jpg
Normal Tissue
Figure 3 Stomach
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/132825_A_6_1.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/132825_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/132825_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (CAPAN-2, HeLa, OE19, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167653-PSCA/subcellular
❮
❯
Specifications
- Immunogen
- Recombinant human PSCA (AA: 1-114) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human PSCA
- Species Reactivity
- Human
- Clone
- 5H5A7
- Molecule Class
- Glycosylphosphatidylinositol (Gpi)-Anchored Cell Surface Protein
- Applications
- ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PSCA; PRO232; Prostate Stem Cell Antigen
- Gene ID
- 8000
- UniProt ID
- O43653
- Long Name
- Prostate stem cell antigen
- Cellular Localization
- Cell membrane; Membrane
- Post Translation Modifications
- Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein
- Protein Refseq
- NP_005663.2
- Function
- May be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro.; May act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro inhibits nicotine-induced signaling probably implicating alpha-3:beta-2- or alpha-7-containing nAChRs.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS7-0425-WR699. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "PSCA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1981z | Mouse Anti-PSCA Recombinant Antibody (clone 24F5) | WB, FC, IF | Mouse IgG1, κ |
| MOB-1641CT | Recombinant Mouse anti-Human PSCA Monoclonal antibody (EML781) | IHC-P, WB | |
| HPAB-1900-FY | Human Anti-PSCA Recombinant Antibody (HPAB-1900-FY) | FC, Inhib, WB, ELISA | Human IgG1 |
| VS-0723-WK291 | Rabbit Anti-PSCA Recombinant Antibody (VS-0723-WK291) | ELISA, FC | Rabbit IgG |
| VS7-0425-WR700 | Mouse Anti-PSCA Recombinant Antibody (clone 3F4E4) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-256CT | Anti-Human PSCA Recombinant Antibody (6B8) | IHC, FC | |
| TAB-257CT | Anti-Human PSCA Recombinant Antibody (1D7) | IHC, FC | |
| TAB-258CT | Anti-Human PSCA Recombinant Antibody (2B3) | IHC, FC | |
| TAB-256CT-S(P) | Anti-Human PSCA Recombinant Antibody scFv Fragment (6B8) | IHC, FC | |
| TAB-257CT-S(P) | Anti-Human PSCA Recombinant Antibody scFv Fragment (1D7) | IHC, FC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-259CT | Anti-Human PSCA Recombinant Antibody (10C5) | IHC, FC | Human antibody |
| TAB-260CT | Anti-Human PSCA Recombinant Antibody (6E4) | IHC, FC | Human antibody |
| TAB-261CT | Anti-Human PSCA Recombinant Antibody (6D1) | IHC, FC | Human antibody |
| TAB-259CT-S(P) | Anti-Human PSCA Recombinant Antibody scFv Fragment (10C5) | IHC, FC | Human antibody |
| TAB-260CT-S(P) | Anti-Human PSCA Recombinant Antibody scFv Fragment (6E4) | IHC, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-262CT | Humanized Anti-PSCA Recombinant Antibody (TAB-262CT) | FuncS | Humanized antibody |
| TAB-262CT-S(P) | Humanized Anti-PSCA Recombinant Antibody; scFv Fragment (TAB-262CT-S(P)) | ELISA, FC | Humanized scFv |
| TAB-262CT-F(E) | Humanized Anti-PSCA Recombinant Antibody; Fab Fragment (TAB-262CT-F(E)) | ELISA, FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0074-CN-S(P) | Mouse Anti-PSCA Recombinant Antibody; scFv Fragment (HPAB-0074-CN-S(P)) | FC | Mouse scFv |
| HPAB-0287CQ-S(P) | Mouse Anti-PSCA Recombinant Antibody (clone mAb 8D11); scFv Fragment | FC, IHC | Mouse scFv |
| HPAB-0288CQ-S(P) | Mouse Anti-PSCA Recombinant Antibody (clone mAb 6F8); scFv Fragment | FC, IHC | Mouse scFv |
| HPAB-1309WJ-S(P) | Mouse Anti-PSCA Recombinant Antibody (clone 6B8); scFv Fragment | ELISA, WB | Mouse scFv |
| HPAB-1900-FY-F(E) | Human Anti-PSCA Recombinant Antibody; Fab Fragment (HPAB-1900-FY-F(E)) | FC, Inhib, WB, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0074-CN-F(E) | Mouse Anti-PSCA Recombinant Antibody; Fab Fragment (HPAB-0074-CN-F(E)) | FC | Mouse Fab |
| HPAB-0286CQ-F(E) | Human Anti-PSCA Recombinant Antibody (clone mAb 5F2); Fab Fragment | FC, IHC | Chimeric (mouse/human) Fab |
| HPAB-0287CQ-F(E) | Mouse Anti-PSCA Recombinant Antibody (clone mAb 8D11); Fab Fragment | FC, IHC | Mouse Fab |
| HPAB-0288CQ-F(E) | Mouse Anti-PSCA Recombinant Antibody (clone mAb 6F8); Fab Fragment | FC, IHC | Mouse Fab |
| HPAB-1900-FY-S(P) | Human Anti-PSCA Recombinant Antibody; scFv Fragment (HPAB-1900-FY-S(P)) | FC, Inhib, WB, ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0023 | APC-H-2Db/Human PSCA (AQMNNRDCL) MHC Tetramer | FCM | |
| MHC-CN0024 | PE-H-2Db/Human PSCA (AQMNNRDCL) MHC Tetramer | FCM | |
| MHC-CN1165 | PE-A*02:01/Human PSCA (QLGEQCWTV) MHC Tetramer | FCM, IHC | |
| MHC-CN1166 | APC-A*02:01/Human PSCA (QLGEQCWTV) MHC Tetramer | FCM, IHC | |
| MHC-CN1167 | FITC-A*02:01/Human PSCA (QLGEQCWTV) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY152 | Mouse Anti-PSCA (clone 6B8) scFv-Fc Chimera | ELISA, Cyt, FC | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC192 | Recombinant Anti-PSCA Vesicular Antibody, EV Displayed (VS-0425-YC192) | ELISA, FC, Cell-uptake |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FC, IHC, FuncS, Inhib, Cyt
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: ELISA
Application: ELISA, Inhib, FuncS
Application: WB, IHC-Fr, IHC-P, ICC, IF
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
