Mouse Anti-RELA Recombinant Antibody (clone 5G6) (CAT#: MOB-0144F)
This product is a mouse antibody that recognizes RELA.
Specific Inquiry
IF
Figure 1 Mouse Anti-RELA Recombinant Antibody (clone 5G6) (MOB-0144F) in IF
Immunofluorescence analysis of Hela cells. 1. BMP-2 polyclonal antibody (green) diluted 1:200 (overnight at 4°C). (Red) Dilute at 1:200 (overnight at 4°C). 2. Goat anti-rabbit Alexa Fluor 488 diluted 1:1000 (room temperature, 50 minutes). Goat Anti Mouse Alexa Fluor594 diluted 1:1000 (room temperature, 50 minutes).
IHC
Figure 2 Mouse Anti-RELA Recombinant Antibody (clone 5G6) (MOB-0144F) in IHC
Immunohistochemical analysis of paraffin-embedded human appendix tissue. 1. NFkB p65 Monoclonal Antibody (5G6) 1:200 dilution (4°C, overnight). 2. Sodium citrate pH 6.0 is used for antibody repair (> 98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30min). The negative control is only used by the secondary antibody.
IHC
Figure 3 Mouse Anti-RELA Recombinant Antibody (clone 5G6) (MOB-0144F) in IHC
Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Sodium citrate pH 6.0 is used for antibody repair (> 98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30min). The negative control is only used by the secondary antibody.
IF
Figure 4 Mouse Anti-RELA Recombinant Antibody (clone 5G6) (MOB-0144F) in IF
Immunofluorescence analysis of human appendix tissue. 1. NFkB p65 Monoclonal Antibody (5G6) (red) 1:200 (4°C, overnight) dilution. 2. The Cy3 labeled secondary antibody was diluted 1:300 (room temperature, 50 minutes). 3. Picture B: DAPI (blue) for 10 minutes. Figure A: Goal. Picture B: DAPI. Picture C: The merger of A+B.
WB
Figure 5 Mouse Anti-RELA Recombinant Antibody (clone 5G6) (MOB-0144F) in WB
1) Hela, 2) Rat heart tissue, 3) Western blot analysis of mouse spleen tissue, diluted 1:2000.
IP
Figure 6 Mouse Anti-RELA Recombinant Antibody (clone 5G6) (MOB-0144F) in IP
1) Hela Cell Lysate 2) IP products: IP dilution 1:200.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human RELA
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 5G6
- Applications
- WB, IHC-P, IF, IP
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunohistologyp-Paraffin Sections, Immunofluorescence, Immunoprecipitation.
Target
- Alternative Names
- RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells; RELA Proto-Oncogene, NF-KB Subunit; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; Nuclear Factor NF-Kappa-B P65 Subunit; NFKB3; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A
- Gene ID
- 5970
- UniProt ID
- Q04206
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "RELA"
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B027(A) | Recombinant Anti-human RELA Intrabody [(D-Arg)9] | WB, CO-IP, FuncS | scFv-(D-Arg)9 |
| IAB-B027(G) | Recombinant Anti-human RELA Intrabody [+36 GFP] | IF, ELISA, FuncS | scFv-(+36GFP) |
| IAB-B027(T) | Recombinant Anti-human RELA Intrabody [Tat] | FC, IF, FuncS | scFv-Tat |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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