Recombinant Mouse Anti-RSV F Antibody (PABC-276) (CAT#: PABC-276)

Figure 1 RSV neutralization

RSV neutralization by antibodies. Palivizumab is the FDA-approved prophylactic antibody that prevents severe RSV disease.

McLellan, J. S., Chen, M., Leung, S., Graepel, K. W., Du, X., Yang, Y., ... & Kumar, A. (2013). Structure of RSV fusion glycoprotein trimer bound to a prefusion-specific neutralizing antibody. Science, 340(6136), 1113-1117.

Figure 2 Antigenicity of RSV prefusion F and postfusion F.

Dots of pre-F, post-F, Heat Inactivated (HI-) pre-F, Formalin Inactivated (FI-) pre-F, RSV or FI-RSV are applied to a nitrocellulose membrane and blotted with antibody 5C4 similarly to a Western blot.

Killikelly, A. M., Kanekiyo, M., & Graham, B. S. (2016). Pre-fusion F is absent on the surface of formalin-inactivated respiratory syncytial virus. Scientific reports, 6, 34108.

Figure 3 Detection of monoclonal antibody binding to avian cells expressing chimeric F proteins. Representative monoclonal antibody binding to chimeric F protein-expressing cells was detected by flow cytometry.

Avian cells transfected with empty vector pCAGGS or pCAGGS containing the gene encoding the F/F, the DS-Cav1 F/F, the pre-F/F, or the post-F/F chimeric protein were incubated with MAb anti-F 5C4 antibody.

Cullen, L. M., Schmidt, M. R., Kenward, S. A., Woodland, R. T., & Morrison, T. G. (2015). Murine immune responses to virus-like particle-associated pre-and postfusion forms of the respiratory syncytial virus F protein. Journal of virology, 89(13), 6835-6847.

Figure 1 Mouse Anti-RSV F Antibody (PABC-276) in SDS-PAGE

SDS-PAGE analysis of PABC-276 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions.

Figure 2 Recombinant Mouse Anti-RSV F Antibody (PABC-276) in HPLC

The purity of PABC-276 was greater than 95% as determined by SEC- HPLC.

Figure 3 Recombinant Mouse Anti-RSV F Antibody (PABC-276) in ELISA

ELISA analysis of PABC-276 was performed by coating with Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag).
The secondary antibody: Goat Anti-Mouse IgG-HRP

Figure 4 Recombinant Mouse Anti-RSV F Antibody (PABC-276) in WB

Western blot analysis of PABC-276 was performed by Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag).
PABC-276 incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Mouse IgG-HRP
Lane1: Reducing antigen (0.1μg)
Lane2: Reducing antigen (0.3μg)
Lane2: Reducing antigen (0.6μg)