This product is a recombinant Human antibody that recognizes RSV F. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Cui, Guanglin, et al. "Preliminary functional and phylogeographic analyses of the 72 nucleotide duplication region in the emerging human respiratory syncytial virus ON1 strain attachment glycoprotein gene." Biomedicine & Pharmacotherapy 123 (2020): 109800.
This study investigates the emerging human respiratory syncytial virus (hRSV) ON1 strain, which is characterized by a 72-nucleotide duplication in the attachment glycoprotein (G) gene. The research utilized a pseudotype lentiviral system to examine the impact of this duplication on hRSV infection. The findings revealed that lentiviral pseudoparticles mimicked the essential functions of the G-protein in facilitating hRSV cell entry and that the deletion of the G-protein duplication significantly reduced the efficiency of pseudotyped lentiviral (pLV) infection. Additionally, the study performed Bayesian skyline and phylogeographic analyses on 199 ON1 sequences, providing insights into the evolutionary and geographical origins of the ON1 strain.
Creative Biolabs provided crucial monoclonal antibodies for this study. The anti-G monoclonal antibody 131-2G (CAT#: TAB-372CT) and the anti-F monoclonal antibody (CAT#: TAB-358CT) were used in Western immunoblotting to confirm the incorporation of G and F proteins into the pseudoparticles. Furthermore, the anti-G monoclonal antibody 131-2G (CAT#: TAB-372CT) was employed in antibody blockade experiments to demonstrate the role of the G protein in hRSV cell entry. These antibodies were essential for verifying the functionality of the pseudotyped lentiviral particles and for the subsequent functional analyses.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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(Creative Biolabs Cat# TAB-358CT, RRID: AB_3111918)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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