Human Anti-RSV F Recombinant Antibody (TAB-358CT) (CAT#: TAB-358CT)

Cui, Guanglin, et al. "Preliminary functional and phylogeographic analyses of the 72 nucleotide duplication region in the emerging human respiratory syncytial virus ON1 strain attachment glycoprotein gene." Biomedicine & Pharmacotherapy 123 (2020): 109800. https://doi.org/10.1016/j.biopha.2019.109800

This research examined the 72-nucleotide duplication in the G gene of the emerging ON1 strain of human respiratory syncytial virus (hRSV). Using pseudotype lentiviral systems, the study directly investigated how this duplication affects viral infection processes. The researchers found that the lentiviral pseudoparticles effectively mimicked key G-protein functions during hRSV cell entry, and notably, deletion of the G-protein duplication significantly decreased infection efficiency. Additionally, phylogeographic analyses of 199 representative ON1 sequences revealed that ON1 isolates likely originated in Europe around 1998, with a nucleotide substitution rate of 2.14 × 10⁻³ substitutions per site per year in the second mucin-like highly variable region of the G-protein gene.
Creative Biolabs supplied anti-G mono-antibody 131-2G (TAB-372CT) and anti-F mono-antibody (TAB-358CT) for this study, which were essential for the immunoblotting and antibody blockade experiments. These antibodies enabled the researchers to validate the incorporation of G and F proteins into pseudoparticles and confirm receptor usage, demonstrating that the lentiviral system accurately replicated authentic hRSV attachment mechanisms. This contribution was instrumental in establishing the enhanced attachment function of the duplicated region in the ON1 G gene, helping explain this strain's rapid global spread and replacement of previously dominant genotypes.