Rabbit Anti-RYK Recombinant Antibody (clone R03-4E1) (CAT#: VS3-FY1945)
The clone R01-0D5 is a rabbit recombinant monoclonal antibody that is meticulously crafted to specifically target human RYK. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We verify the QC result of the antibody through western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ICC
Figure 1 Anti-RYK Antibody (VS3-FY1945) in ICC.
Immunocytochemistry staining of RYK (green) in hela using the anti-RYK antibody. Nuclear DNA was labeled with DAPI (shown in blue).
IHC
Figure 2 Anti-RYK Antibody (VS3-FY1945) in IHC.
Immunohistochemistry staining of paraffin-embedded human lung cancer using the anti-RYK antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human RYK
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human RYK
- Species Reactivity
- Human
- Clone
- R03-4E1
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 68 kDa, while the observed molecular weight stands at 72 kDa.
- Related Disease
- Human lung cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Receptor-Like Tyrosine Kinase; JTK5A Protein Tyrosine Kinase; EC 2.7.10.1; JTK5A; RYK Receptor-Like Tyrosine Kinase; Tyrosine-Protein Kinase RYK
- Gene ID
- 6259
- UniProt ID
- P34925
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family.
- Cellular Localization
- Cytoplasm, Membrane, Nucleus
- Post Translation Modifications
- Proteolytically cleaved, in part by presenilin, in response to WNT3 stimulation.
Cleavage occurs during neuronal differentiation.
(P34925-RYK_HUMAN)
Glycosylation at Asn139, Asn174, Asn178, Asn182, and Asn209 (NX_P34925 [NX_P34925-1])
Modification sites at PhosphoSitePlus (P34925)
Glycosylation from GlyGen (P34925) 5 sites
- Protein Refseq
- NP_001005861.1; NP_002949.2
- Function
- May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Human Antibody Products
IgG Antibody Products
Rabbit Monoclonal Antibody Products
ScFv Antibody Products
Fab Antibody Products
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-375CT | Anti-Human RYK Recombinant Antibody (RWD1) | WB, IHC, ELISA | Human antibody |
| TAB-375CT-S(P) | Anti-Human RYK Recombinant Antibody scFv Fragment (RWD1) | WB, ELISA, BL. IHC, IP, FC | Human antibody |
| TAB-375CT-F(E) | Anti-Human RYK Recombinant Antibody Fab Fragment (RWD1) | WB, ELISA, BL. IHC, IP, FC | Human antibody |
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