Rabbit Anti-SHC1 Recombinant Antibody (clone R02-1J3) (CAT#: VS3-XY3505)
This product is a recombinant rabbit antibody targeting the SHC1 (SHC adaptor protein 1), which plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. This antibody recognizes and binds to human SHC1. It also has cross-reactivity against mouse and rat SHC1. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50-1/100, 1/50-1/100 and 1/50-1/200, respectively.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5374/if_selected.jpg

(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/if_selected.jpg

(Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1844/ihc_selected.jpg

(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5374/ihc_selected.jpg

(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/ihc_selected.jpg

(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/36379_A_6_6.jpg

(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/36379_A_7_8.jpg

(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/36379_A_7_3.jpg

(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/36379_A_7_5.jpg

(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160691-SHC1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SHC1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-1J3
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Breast Cancer; Multiple Endocrine Neoplasia, Type Iia
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- SHC; SHCA
- Gene ID
- 6464
- UniProt ID
- P29353
- Long Name
- SHC adaptor protein 1
- Cellular Localization
- Cytoplasm, Cell junction, focal adhesion
- Post Translation Modifications
- Phosphorylated by activated epidermal growth factor receptor.
Phosphorylated in response to FLT4 and KIT signaling.
Isoform p46Shc and isoform p52Shc are phosphorylated on tyrosine residues of the Pro-rich domain.
Isoform p66Shc is phosphorylated on Ser-36 by PRKCB upon treatment with insulin, hydrogen peroxide or irradiation with ultraviolet light (By similarity).
- Protein Refseq
- XP_005245508.1
- Function
- Signaling adapter that couples activated growth factor receptors to signaling pathways.
Participates in a signaling cascade initiated by activated KIT and KITLG/SCF.
Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SHC1"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-3923z | Mouse Anti-SHC1 Recombinant Antibody (clone 26H5) | WB, FC, IF | Mouse IgG1 |
MOB-1856MZ | Recombinant Mouse Anti-Human SHC1 Antibody (clone 3F4) | IHC-P, WB | Mouse antibody |
VS3-QX1008 | Mouse Anti-SHC1 Recombinant Antibody (clone 1C4-F0-E4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
VS3-QX1009 | Mouse Anti-SHC1 Recombinant Antibody (clone 7C-7F) | WB, ICC, FC | Mouse IgG1 |
VS7-0425-WR852 | Mouse Anti-SHC1 Recombinant Antibody (clone 2F7C7) | WB, ICC, FC | Mouse IgG1 |
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