Rabbit Anti-SHC1 Recombinant Antibody (clone R02-1J3)
CAT#: VS3-XY3505
This product is a recombinant rabbit antibody targeting the SHC1 (SHC adaptor protein 1), which plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. This antibody recognizes and binds to human SHC1. It also has cross-reactivity against mouse and rat SHC1. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50-1/100, 1/50-1/100 and 1/50-1/200, respectively.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5374/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human placenta
Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1844/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5374/ihc_selected.jpg
Normal Tissue
Figure 5 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/ihc_selected.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/36379_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/36379_A_7_8.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/36379_A_7_3.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/36379_A_7_5.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160691-SHC1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SHC1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-1J3
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Breast Cancer; Multiple Endocrine Neoplasia, Type Iia
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- SHC; SHCA
- Gene ID
- 6464
- UniProt ID
- P29353
- Long Name
- SHC adaptor protein 1
- Cellular Localization
- Cytoplasm, Cell junction, focal adhesion
- Post Translation Modifications
- Phosphorylated by activated epidermal growth factor receptor.
Phosphorylated in response to FLT4 and KIT signaling.
Isoform p46Shc and isoform p52Shc are phosphorylated on tyrosine residues of the Pro-rich domain.
Isoform p66Shc is phosphorylated on Ser-36 by PRKCB upon treatment with insulin, hydrogen peroxide or irradiation with ultraviolet light (By similarity).
- Protein Refseq
- XP_005245508.1
- Function
- Signaling adapter that couples activated growth factor receptors to signaling pathways.
Participates in a signaling cascade initiated by activated KIT and KITLG/SCF.
Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals.
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Related Diseases
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Hepatocellular Carcinoma
Non-small Cell Lung Cancer
Alcoholism
Related Signaling Pathways
ErbB Signaling Pathway
MAPK Signaling Pathway
PPAR Signaling Pathway
Ras Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SHC1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1856MZ | Recombinant Mouse Anti-Human SHC1 Antibody (clone 3F4) | IHC-P, WB | Mouse antibody |
| VS3-QX1008 | Mouse Anti-SHC1 Recombinant Antibody (clone 1C4-F0-E4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX1009 | Mouse Anti-SHC1 Recombinant Antibody (clone 7C-7F) | WB, ICC, FC | Mouse IgG1 |
| VS3-XY1419 | Mouse Anti-SHC1 Recombinant Antibody (clone 2C1) | ELISA, WB, FC, ICC | Mouse IgG1 |
| VS7-0425-WR852 | Mouse Anti-SHC1 Recombinant Antibody (clone 2F7C7) | WB, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3260 | Hi-Affi™ Recombinant Rabbit Anti-SHC1 Monoclonal Antibody (DS3260AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6563 | Anti-SHC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6564 | Anti-Mouse SHC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6565 | Anti-Rat SHC1 Immunohistochemistry Kit | IHC |
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