Rabbit Anti-SMAD3 (pS213) Recombinant Antibody (VS3-WK2184)
CAT#: VS3-WK2184
This product is a rabbit recombinant antibody that is specific to phosphorylated SMAD3. The target of this antibody is phospho-SMAD3 (Ser213). It can be used in WB analysis at a dilution of 1/500-1/2000 and ELISA analysis at a dilution of 1/10000-20000.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46386/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8094/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human parathyroid gland
Immunohistochemical staining of human parathyroid gland shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8094/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_7_2_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_8_8.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166949-SMAD3
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Specifications
- Immunogen
- Synthesized phospho derived from human Smad3 (phospho-Ser213)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser213 of human, mouse and rat SMAD3
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS containing 50% Glycerol and 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/2000
ELISA: 1/10000-1/20000
Target
- Alternative Names
- SMAD Family Member 3; Mothers Against DPP Homolog 3; MAD Homolog 3; JV15-2; HMAD-3; HSMAD3; MADH3; MAD3; MAD, Mothers Against Decapentaplegic Homolog 3 (Drosophila); SMAD, Mothers Against DPP Homolog 3 (Drosophila); MAD, Mothers Against Decapentaplegic Homolog 3; Mothers Against Decapentaplegic Homolog 3
- Gene ID
- 4088
- UniProt ID
- P84022
- Long Name
- SMAD Family Member 3
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Related Signaling Pathways
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Related Diseases
Pancreatic Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "SMAD3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3147z | Mouse Anti-SMAD3 Recombinant Antibody (clone 24F4) | WB, ELISA, IHC | Mouse IgG1, κ |
| MRO-2380-CN | Recombinant Rabbit Anti-SMAD3 (phosphorylated Ser423/S425) Monoclonal Antibody (CBACN-665) | WB, IF, IHC | Rabbit IgG |
| MOB-0198F | Mouse Anti-Smad3 Recombinant Antibody (clone 1D9) | IHC-P, IF | Mouse IgG |
| VS3-WK1563 | Rabbit Anti-SMAD3 Polyclonal Antibody | WB, ELISA | Rabbit IgG |
| VS3-WK2193 | Mouse Anti-SMAD3 (pS425) Recombinant Antibody (VS3-WK2193) | IHC-P, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3322 | Hi-Affi™ Recombinant Rabbit Anti-SMAD3 Monoclonal Antibody (DS3322AB) | WB, ICC, IHC-P, IP, sELISA | IgG |
| MOR-0052-FY | Rabbit Anti-SMAD3 Recombinant Antibody (clone AFY0023) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1125 | AbPlus™ Anti-SMAD3 Magnetic Beads (VS-0724-YC1125) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY485 | Rabbit Anti-NHP SMAD3 Recombinant Antibody (clone E.980.9) | WB, IF, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY199 | Human Anti-SMAD3 scFv-Fc Chimera (VS-0325-FY199) | ELISA, IF | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1078 | CytoStream™ Rabbit Anti-SMAD3 Recombinant Antibody (VS13-YC1078) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2106 | Anti-SMAD3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6718 | Anti-Rat SMAD3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6719 | Anti-Mouse SMAD3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6717 | Anti-Human SMAD3 Immunohistochemistry Kit | IHC |
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