Mouse Anti-SMAD3 Recombinant Antibody (VS3-WK1363)
CAT#: VS3-WK1363
This product is a mouse monoclonal antibody that recognizes SMAD3.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46386/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8094/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human parathyroid gland
Immunohistochemical staining of human parathyroid gland shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8094/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_7_2_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_8_8.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166949-SMAD3
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Specifications
- Immunogen
- Recombinant protein of Smad3
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Human SMAD3
- Species Reactivity
- Human, Rat, Mouse
- Applications
- IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C for 1 year.Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry-Paraffin (1:100-200) and Western Blot (1000-2000).
Target
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Related Signaling Pathways
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Related Diseases
Pancreatic Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
See other products for "SMAD3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3322 | Hi-Affi™ Recombinant Rabbit Anti-SMAD3 Monoclonal Antibody (DS3322AB) | WB, ICC, IHC-P, IP, sELISA | IgG |
| MOR-0052-FY | Rabbit Anti-SMAD3 Recombinant Antibody (clone AFY0023) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1404-CN | Recombinant Rabbit Anti-SMAD3 Monoclonal Antibody (CBACN-512) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-2192-CN | Rabbit Anti-SMAD3 Polyclonal Antibody (MRO-2192-CN) | IF, IHC, FC | Rabbit IgG |
| MRO-2193-CN | Rabbit Anti-SMAD3 Polyclonal Antibody (MRO-2193-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-FY1358 | Recombinant Rabbit Anti-SMAD3 Antibody (clone R02-1I2) | WB, IP | Rabbit IgG |
| VS3-WK2184 | Rabbit Anti-SMAD3 (pS213) Recombinant Antibody (VS3-WK2184) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1125 | AbPlus™ Anti-SMAD3 Magnetic Beads (VS-0724-YC1125) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY485 | Rabbit Anti-NHP SMAD3 Recombinant Antibody (clone E.980.9) | WB, IF, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY199 | Human Anti-SMAD3 scFv-Fc Chimera (VS-0325-FY199) | ELISA, IF | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1078 | CytoStream™ Rabbit Anti-SMAD3 Recombinant Antibody (VS13-YC1078) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2106 | Anti-SMAD3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6718 | Anti-Rat SMAD3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6719 | Anti-Mouse SMAD3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6717 | Anti-Human SMAD3 Immunohistochemistry Kit | IHC |
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