Mouse Anti-SPP1 Recombinant Antibody (clone 1A12) (CAT#: HPAB-1363-FY)

Figure 1 In vivo study of anti-OPN antibodies.

Lung metastasis of breast cancer xenograft model. Female nude mice were given injections of 5 × 10 6 MDA-MB-435s cells into the MFP. Representative lung tissue sections of hematoxylin and eosin staining from the nude mice after MFP injection of MDA-MB-435s cells with or without anti-OPN antibody treatment. Arrows indicate tumor metastasis sites. Anti-OPN antibodies treatment inhibited lung metastasis in the orthotopic model.

Peng, L., Guo, Y., Zhou, Y., Dai, J., & Wang, H. (2009). Inhibition of Breast Cancer Metastasis and Angiogenesis by Antiosteopontin Single-Chain Fv-Fc Fusion Protein. Neoplasia, 11(5), 509-519.

Figure 2 Antiangiogenesis effect of anti-OPN antibodies.

In some experiments, 1 μg of 1A12 and 2H8 was added simultaneously with OPN to examine its ability to inhibit the OPN-induced angiogenic response. Neovascularization was observed in OPN and VEGF group, whereas no vascular response was observed in control CAM with PBS.

Peng, L., Guo, Y., Zhou, Y., Dai, J., & Wang, H. (2009). Inhibition of Breast Cancer Metastasis and Angiogenesis by Antiosteopontin Single-Chain Fv-Fc Fusion Protein. Neoplasia, 11(5), 509-519.

Figure 3 The construction and biopanning of phage antibody library.

Competitive ELISA was performed to determine the competitive binding inhibition between phage scFv and scFv-Fc. Inhibition of phage scFv binding to OPN was observed by using scFv-Fc fusion protein as a competitor. The bound phages were detected by ELISA with HRPconjugated anti-M13 antibody.

Peng, L., Guo, Y., Zhou, Y., Dai, J., & Wang, H. (2009). Inhibition of Breast Cancer Metastasis and Angiogenesis by Antiosteopontin Single-Chain Fv-Fc Fusion Protein. Neoplasia, 11(5), 509-519.