Rabbit Anti-SYK (pY348) Recombinant Antibody (VS3-WK2182) (CAT#: VS3-WK2182)
This product is a rabbit recombinant antibody that is specific to phosphorylated SYK. The target of this antibody is phospho-SYK (Tyr348). It can be used in WB analysis at a dilution of 1/500-1/2000 and ELISA analysis at a dilution of 1/10000-20000.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1384/1472_A1_6_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1384/1472_A1_6_selected.jpg
Figure 2 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_B_9_5.jpg
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_B_9_5.jpg
Figure 3 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_8_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_8_3.jpg
Figure 4 Liver
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_9_4.jpg
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_9_4.jpg
Figure 5 Kidney
(Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_5.jpg
(Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_5.jpg
Figure 6 Testis
(Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_6.jpg
(Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_8.jpg
Figure 8 Tonsil
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Squamous epithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Squamous epithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (Daudi, HEL, HL-60, HMC-1, REH, THP-1, U-698, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165025-SYK/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165025-SYK/subcellular
Specifications
- Immunogen
- Synthesized phospho derived from human SYK (phospho-Tyr348)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Tyr348 of human, mouse and rat SYK
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS containing 50% Glycerol and 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/2000
ELISA: 1/10000-1/20000
Target
- Alternative Names
- IMD82; p72-Syk
- Gene ID
- 6850
- UniProt ID
- P43405
- Long Name
- Spleen associated tyrosine kinase
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
BCR Signaling Pathway
FcεR1 Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-937-F(E) | Recombinant Anti-human SYK Antibody Fab Fragment | IF, FACS, FuncS | Fab |
| MHH-937-F(E) | Recombinant Human Anti-human SYK Antibody Fab Fragment | FC, ELISA, FuncS | Fab |
| HPAB-0225-YC-F(E) | Rabbit Anti-SYK Recombinant Antibody; Fab Fragment (HPAB-0225-YC-F(E)) | IHC, FuncS | Rabbit Fab |
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