Rabbit Anti-SYK (pY348) Recombinant Antibody (VS3-WK2182)
CAT#: VS3-WK2182
This product is a rabbit recombinant antibody that is specific to phosphorylated SYK. The target of this antibody is phospho-SYK (Tyr348). It can be used in WB analysis at a dilution of 1/500-1/2000 and ELISA analysis at a dilution of 1/10000-20000.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1384/1472_A1_6_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_8.jpg
Normal Tissue
Figure 8 Tonsil
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Squamous epithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (Daudi, HEL, HL-60, HMC-1, REH, THP-1, U-698, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165025-SYK/subcellular
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Specifications
- Immunogen
- Synthesized phospho derived from human SYK (phospho-Tyr348)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Tyr348 of human, mouse and rat SYK
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS containing 50% Glycerol and 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/2000
ELISA: 1/10000-1/20000
Target
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Related Signaling Pathways
BCR Signaling Pathway
FcεR1 Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "SYK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-937-F(E) | Recombinant Anti-human SYK Antibody Fab Fragment | IF, FACS, FuncS | Fab |
| MHH-937-F(E) | Recombinant Human Anti-human SYK Antibody Fab Fragment | FC, ELISA, FuncS | Fab |
| HPAB-0225-YC-F(E) | Rabbit Anti-SYK Recombinant Antibody; Fab Fragment (HPAB-0225-YC-F(E)) | IHC, FuncS | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-937-S(P) | Recombinant Anti-human SYK Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| MHH-937-S(P) | Recombinant Human Anti-human SYK Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| HPAB-0225-YC-S(P) | Rabbit Anti-SYK Recombinant Antibody; scFv Fragment (HPAB-0225-YC-S(P)) | IHC, FuncS | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-937 | Recombinant Human Anti-human SYK Antibody | ELISA, WB, Dot, FuncS | IgG |
| MOB-1977MZ | Recombinant Mouse Anti-Human SYK Antibody (clone 8H5) | WB | Mouse antibody |
| HPAB-0225-YC | Rabbit Anti-SYK Recombinant Antibody (HPAB-0225-YC) | IHC, FuncS | Rabbit IgG |
| VS3-WK49 | Mouse Anti-SYK Recombinant Antibody (clone E6-G11) | WB, ELISA | Mouse IgG1 |
| VS3-WK1561 | Rabbit Anti-SYK Polyclonal Antibody | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3476 | Hi-Affi™ Recombinant Rabbit Anti-SYK Monoclonal Antibody (DS3476AB) | ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY47 | Rabbit Anti-SYK (clone MIL81-1-8) scFv-Fc Chimera | IHC | Rabbit IgG, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7041 | Anti-SYK Immunohistochemistry Kit | IHC | |
| VS-0525-XY7042 | Anti-Mouse SYK Immunohistochemistry Kit | IHC |
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