Rabbit Anti-SYK (clone MIL81-1-8) scFv-Fc Chimera
CAT#: VS-0425-FY47
The anti-SYK scFv-Fc fusion protein targets Spleen tyrosine kinase (SYK), a key enzyme in immune signaling. This engineered protein combines a single-chain variable fragment (scFv) for specific SYK binding with an Fc domain to enhance stability and effector functions. Its primary mechanism involves inhibiting SYK activity, thereby disrupting downstream signaling pathways involved in inflammation and autoimmune responses. This disruption can mitigate conditions like rheumatoid arthritis and allergic reactions. The Fc portion extends the protein's half-life, improving its therapeutic potential.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1384/1472_A1_6_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_8.jpg
Normal Tissue
Figure 8 Tonsil
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Squamous epithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (Daudi, HEL, HL-60, HMC-1, REH, THP-1, U-698, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165025-SYK/subcellular
❮
❯
Specifications
- Immunogen
- The immunogen used to generate the MIL81-1-8 antibody was a synthetic phosphopeptide corresponding to residues surrounding Tyr525/526 of human SYK.
- Host Species
- Rabbit
- Type
- Rabbit IgG, scFv-Fc
- Specificity
- The MIL81-1-8 clone is reactive to phosphorylated SYK (pSYK), specifically at tyrosine residues 525 and 526 (pSYK Y525/526).
- Species Reactivity
- Human
- Clone
- MIL81-1-8
- Applications
- Immunohistochemistry
- Related Disease
- Autoimmune disease, Alzheimer's disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Immunohistochemistry: 3.5 μg/mL
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- IMD82; p72-Syk
- Gene ID
- 6850
- UniProt ID
- A8K4G2
- Long Name
- Spleen Associated Tyrosine Kinase
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm
- Post Translation Modifications
- Ubiquitinated by CBLB after BCR activation; which promotes proteasomal degradation.
Autophosphorylated.
Ubiquitination at Lys517
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005252204.1; XP_011517248.1; XP_047279765.1; XP_047279766.1; XP_047279767.1
- Function
- Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR).
Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development.
Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0425-FY47. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
BCR Signaling Pathway
FcεR1 Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SYK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-937-F(E) | Recombinant Anti-human SYK Antibody Fab Fragment | IF, FACS, FuncS | Fab |
| MHH-937-F(E) | Recombinant Human Anti-human SYK Antibody Fab Fragment | FC, ELISA, FuncS | Fab |
| HPAB-0225-YC-F(E) | Rabbit Anti-SYK Recombinant Antibody; Fab Fragment (HPAB-0225-YC-F(E)) | IHC, FuncS | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-937-S(P) | Recombinant Anti-human SYK Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| MHH-937-S(P) | Recombinant Human Anti-human SYK Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| HPAB-0225-YC-S(P) | Rabbit Anti-SYK Recombinant Antibody; scFv Fragment (HPAB-0225-YC-S(P)) | IHC, FuncS | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-937 | Recombinant Human Anti-human SYK Antibody | ELISA, WB, Dot, FuncS | IgG |
| HPAB-0225-YC | Rabbit Anti-SYK Recombinant Antibody (HPAB-0225-YC) | IHC, FuncS | Rabbit IgG |
| VS3-WK2182 | Rabbit Anti-SYK (pY348) Recombinant Antibody (VS3-WK2182) | WB, ELISA | Rabbit IgG |
| VS7-0425-WR940 | Mouse Anti-SYK Recombinant Antibody (clone 2G11E6) | ICC, FC | Mouse IgG1 |
| VS7-0425-WR941 | Mouse Anti-SYK Recombinant Antibody (clone 3D1G10) | WB, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3476 | Hi-Affi™ Recombinant Rabbit Anti-SYK Monoclonal Antibody (DS3476AB) | ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7041 | Anti-SYK Immunohistochemistry Kit | IHC | |
| VS-0525-XY7042 | Anti-Mouse SYK Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: ELISA, FuncS, IHC, IF, FC, ADCC
Application: ELISA, IHC, FC, IP, IF, FuncS
-3.jpg)
Application: ELISA, WB
Application: ELISA, FC
Application: ELISA, FuncS
Application: ELISA, Inhib, FuncS
Application: ELISA, FuncS, RIA, IP, IF, IHC, IA, Block
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
