Mouse Anti-TCF4 Recombinant Antibody (clone 7B1B3)
CAT#: VS7-0425-WR958
This product is a mouse IgG2b antibody that binds to human TCF4. The antibody 7B1B3 recognizes specific amino acid domain of human TCF4 (AA: 518-667). It can be used for FC assay.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20722/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25958/55485_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25958/55485_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25958/55485_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25958/55485_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25958/55485_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25958/55485_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HDLM-2, SH-SY5Y)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196628-TCF4
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Specifications
- Immunogen
- Recombinant human TCF4 (AA: 518-667) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human TCF4
- Species Reactivity
- Human
- Clone
- 7B1B3
- Molecule Class
- Class I Bhlh Family
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Pitt-Hopkins syndrome (PTHS); Corneal dystrophy, Fuchs endothelial, 3 (FECD3)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- TCF4; SEF2; Transcription Factor 4; E2-2; PTHS; ITF2; BHLHb19; TCF-4; SEF2-1; Class B Basic Helix-Loop-Helix Protein 19; Immunoglobulin Transcription Factor 2; SEF2-1A; SEF2-1B; SL3-3 Enhancer Factor 2; ITF-2
- Gene ID
- 6925
- UniProt ID
- P15884
- Long Name
- Transcription factor 4
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001077431.1
- Function
- Activator; DNA-binding
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Related Diseases
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Related Signaling Pathways
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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Secondary Antibody
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Reconstitution Buffer
- CAT
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Recommended Dilution Buffer
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- Product Name
See other products for "TCF4"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3599z | Mouse Anti-TCF4 Recombinant Antibody (clone 32H10) | WB, ELISA, FC | Mouse IgG1 |
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| VS3-XY1491 | Mouse Anti-TCF4 Recombinant Antibody (clone 4D4C4) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1147 | CytoStream™ Rabbit Anti-TCF4 Recombinant Antibody (VS13-YC1147) | WB, IHC-P, FC, ChIP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2256 | Anti-TCF4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7137 | Anti-Human TCF4 Immunohistochemistry Kit | IHC |
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