Mouse Anti-TFRC Recombinant Antibody (VS3-CJ313)
CAT#: VS3-CJ313
This product is a mouse antibody that recognizes TFRC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to endosomes & lysosomes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28598/546_F8_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/207_B2_1_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/208_B2_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_3.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Pachytene spermatocytes
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_6_6.jpg
Normal Tissue
Figure 7 Bone marrow
Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_6_4.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000072274-TFRC
❮
❯
Specifications
- Immunogen
- KLH-conjugated synthetic peptide between 649-677 amino acids from human TFRC
- Host Species
- Mouse
- Type
- Mouse IgM
- Specificity
- Human TFRC
- Species Reactivity
- Human
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000).
Target
- Alternative Names
- T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46
- Gene ID
- 7037
- UniProt ID
- P02786
- Sequence Similarities
- Belongs to the peptidase M28 family. M28B subfamily.
- Cellular Localization
- Cell membrane, Membrane, Secreted
- Post Translation Modifications
- Stearoylated by ZDHHC6 which inhibits TFRC-mediated activation of the JNK pathway and promotes mitochondrial fragmentation (PubMed:26214738).
Stearoylation does not affect iron uptake (PubMed:26214738).
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
- Protein Refseq
- NP_001121620.1; NP_001300894.1; NP_001300895.1
- Function
- Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738).
Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity).
A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738).
When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738).
When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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|---|---|---|---|
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| TAB-0464CL-S(P) | Mouse Anti-TFRC Recombinant Antibody; scFv Fragment (TAB-0464CL-S(P)) | FuncS | Mouse scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0467CL-F(E) | Human Anti-TFRC Recombinant Antibody; Fab Fragment (TAB-0467CL-F(E)) | FuncS | Human Fab |
| TAB-619CT | Human Anti-TFRC Recombinant Antibody (TAB-619CT) | ELISA, WB | Human antibody |
| TAB-620CT | Human Anti-TFRC Recombinant Antibody (TAB-620CT) | ELISA, WB | Human antibody |
| TAB-621CT | Anti-Human TfR Recombinant Antibody (LUCA31) | ELISA, WB, FC | Human antibody |
| TAB-623CT | Anti-Human TfR Recombinant Antibody (3TF12) | WB | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-611CT | Humanized Anti-TFRC Recombinant Antibody (TAB-611CT) | ELISA | Humanized IgG |
| TAB-612CT | Humanized Anti-TFRC Recombinant Antibody (TAB-612CT) | ELISA | Humanized IgG |
| TAB-613CT | Humanized Anti-TFRC Recombinant Antibody (TAB-613CT) | ELISA | Humanized IgG |
| TAB-614CT | Humanized Anti-TFRC Recombinant Antibody (TAB-614CT) | ELISA | Humanized IgG |
| TAB-611CT-S(P) | Humanized Anti-TFRC Recombinant Antibody; scFv Fragment (TAB-611CT-S(P)) | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-615CT | Anti-Human TfR Recombinant Antibody (6-90) | ELISA, WB, IP |
| CAT | Product Name | Application | Type |
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| NEUT-1999CQ | Rat Anti-Tfrc Recombinant Antibody (clone RI7217) | FC, ICC, IP, WB, Block | Rat IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3370 | PE-DRB1*13:02/Human TFRC (NPGGYVAYSKAATVTGKL) MHC Tetramer | FCM | |
| MHC-LC3371 | PE-DRB1*13:02/Human TFRC (NPGGYVAYSKAATVTG) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0376-CN | Mouse Anti-TFRC Recombinant Antibody (HPAB-0376-CN) | ELISA, FC | Mouse IgG |
| HPAB-0377-CN | Mouse Anti-TFRC Recombinant Antibody (HPAB-0377-CN) | ELISA, FC | Mouse IgG |
| HPAB-0378-CN | Mouse Anti-TFRC Recombinant Antibody (HPAB-0378-CN) | ELISA, FC | Mouse IgG |
| HPAB-0379-CN | Human Anti-TFRC Recombinant Antibody (HPAB-0379-CN) | ELISA, FC | Humanized IgG |
| HPAB-0380-CN | Mouse Anti-TFRC Recombinant Antibody (HPAB-0380-CN) | ELISA, FC, IF, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0376-CN-F(E) | Mouse Anti-TFRC Recombinant Antibody; Fab Fragment (HPAB-0376-CN-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-0377-CN-F(E) | Mouse Anti-TFRC Recombinant Antibody; Fab Fragment (HPAB-0377-CN-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-0378-CN-F(E) | Mouse Anti-TFRC Recombinant Antibody; Fab Fragment (HPAB-0378-CN-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-0379-CN-F(E) | Human Anti-TFRC Recombinant Antibody; Fab Fragment (HPAB-0379-CN-F(E)) | ELISA, FC | Humanized Fab |
| HPAB-0380-CN-F(E) | Mouse Anti-TFRC Recombinant Antibody Fab Fragment (HPAB-0380-CN-F(E)) | ELISA, FC, IF, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-035LC-S(P) | Rat Anti-TFRC Recombinant Antibody (clone 9E4); scFv Fragment | FuncS, IHC, FC | Rat scFv |
| FAMAB-0281-YC-S(P) | Mouse Anti-TFRC Recombinant Antibody (clone OX26); scFv Fragment | ELISA | Mouse scFv |
| HPAB-597-FY-S(P) | Human Anti-TFRC Recombinant Antibody; scFv Fragment (HPAB-597-FY-S(P)) | ELISA | Humanized scFv |
| HPAB-2579LY-S(P) | Mouse Anti-TFRC Recombinant Antibody; scFv Fragment (HPAB-2579LY-S(P)) | ELISA | Mouse scfv |
| HPAB-AP469-YC-S(P) | Human Anti-TFRC Recombinant Antibody (clone c5D7); scFv Fragment | FC, WB, Cyt, Inhib, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1481 | AbPlus™ Anti-TFRC Magnetic Beads (VS-0724-YC1481) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY205 | CytoStream™ Mouse Anti-TFRC Recombinant Antibody (clone OX-26) | FC, IHC, IP | Mouse IgG2a, kappa |
| VS-0225-XY206 | CytoStream™ Rat Anti-TFRC Recombinant Antibody (clone RI7217) | FC | Rat IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC571 | Recombinant Anti-TFRC Vesicular Antibody, EV Displayed (VS-0425-YC571) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7200 | Anti-Mouse TFRC Immunohistochemistry Kit | IHC | |
| VS-0525-XY7201 | Anti-Rat TFRC Immunohistochemistry Kit | IHC |
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