Mouse Anti-TFRC Recombinant Antibody (VS3-CJ313)

Name: Mouse Anti-TFRC Recombinant Antibody (VS3-CJ313)
SKU: VS3-CJ313
Rating: 5 (1 reviews)

CAT#: VS3-CJ313

This product is a mouse antibody that recognizes TFRC.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to endosomes & lysosomes.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28598/546_F8_1_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/207_B2_1_blue_red_green.jpg

Subcellular Location Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/208_B2_1_blue_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Endothelial cells
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_9_5.jpg

Normal Tissue Figure 5 Colon

Glandular cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_3.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Pachytene spermatocytes
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_6_6.jpg

Normal Tissue Figure 7 Bone marrow

Hematopoietic cells
Staining:

High

Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_6_4.jpg

Normal Tissue Figure 8 Lymph node

Germinal center cells
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000072274-TFRC

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Specifications

Immunogen
KLH-conjugated synthetic peptide between 649-677 amino acids from human TFRC

Host Species
Mouse

Type
Mouse IgM

Specificity
Human TFRC

Species Reactivity
Human

Applications
WB

Conjugate
Unconjugated

Product Property

Purification
Affinity purified

Purity
>95% as determined by SDS-PAGE

Buffer
PBS

Preservative
0.09% sodium azide

Storage
Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
This antibody has been tested for use in Western Blot (1:1000).

Target

Alternative Names
T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46

Gene ID
7037

UniProt ID
P02786

Sequence Similarities
Belongs to the peptidase M28 family. M28B subfamily.

Cellular Localization
Cell membrane, Membrane, Secreted

Post Translation Modifications
Stearoylated by ZDHHC6 which inhibits TFRC-mediated activation of the JNK pathway and promotes mitochondrial fragmentation (PubMed:26214738).
Stearoylation does not affect iron uptake (PubMed:26214738).
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

Protein Refseq
NP_001121620.1; NP_001300894.1; NP_001300895.1

Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738).
Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity).
A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738).
When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738).
When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738).

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-CJ313.pdf

MSDS

MSDS - VS3-CJ313.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Secondary Antibody

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